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| Effect of the Xingnao Kaiqiao acupuncture therapy on expression of p-Akt (Ser473) and neurocyte apoptosis in the hippocampus of rats with cerebral ischemia/reperfusion injury |
| Hits 1534 Download times 1336 Received:June 24, 2017 |
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| DOI
10.11656/j.issn.1672-1519.2018.08.15 |
| Key Words
Xingnao Kaiqiao acupuncture therapy;cerebral ischemia reperfusion;apoptosis;PI3K/Akt signaling pathway |
| Author Name | Affiliation | | HAN Lin | Acupuncture Department, The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | GAO Yang | Acupuncture Department, The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | WANG Xuhui | Acupuncture Department, The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | ZHANG Yanan | Acupuncture Research Institute, The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | ZHANG Xiangyu | Acupuncture Department, The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | MENG Lina | Acupuncture Department, The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | ZHU Qian | Acupuncture Department, The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
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| Abstract
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| [Objective] To observe the effects of the Xingnao Kaiqiao acupuncture therapy on expressions of p-Akt (Ser473) and neurocyte apoptosis in the hippocampus of rats with cerebral ischemia/reperfusion injury.[Methods] The 78 Sprague-Dawley rats were randomly divided into three groups, the sham-operation group, the I/R group and the electroacupuncture group, 26 rats in each group. The cerebral ischemia reperfusion model was established by Longa's suture method. In electroacupuncture group, acupuncture was performed with Xingnao Kaiqiao method at main acupoints of Neiguan (PC6), Renzhong (DU26), Sanyinjiao (SP6) and the electric acupuncture apparatus was connected to ipsilateral Neiguan (PC6) and Sanyinjiao (SP6). Then acupunctures were followed twice daily for 3 days. Neurological deficits were assessed by ethologic scoring according to Zausinger's neurologic assessment scales, the 2, 3, 4-triphenyltetrazolium chloride (TTC) staining was used to detect infarct volume, pathological brain damage was estimated by performing hematoxylin and eosin staining. In addition, neurocyte apoptosis in the hippocampus was detected by flow cytometry and the protein expressions of p-Akt (Ser473), Bcl-2 and Bax in hippocampus were measured by Western blot.[Results] In comparison with the model group, the neurological deficits scores of the electroacupuncture group increased and the infarction volume decreased obviously (P<0.01), the pathological damage alleviated significantly. At the same time, compared with model group, the hippocampal neuron's total apoptosis rate obviously decreased (P<0.05)in electroacupuncture group accompanied with the increments of protein expressions of p-Akt (Ser473), Bcl-2 and the ratio of Bcl-2/Bax (P<0.05, P<0.01), and with the reduction of protein expression of Bax (P<0.01).[Conclusion] Xingnao Kaiqiao acupuncture therapy can significantly improve neurological function and reduce neurocyte apoptosis in rats with focal cerebral ischemia reperfusion injury. The protective effects of acupuncture on the brain may be achieved by activating the PI3K/Akt signaling pathway. |
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