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| Researching the mechanism of rapamycin on area of plaque in atherosclerotic mice |
| Hits 1711 Download times 1335 Received:May 23, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2018.08.16 |
| Key Words
atherosclerosis;plaques;rapamycin |
| Author Name | Affiliation | E-mail | | WANG Qinglong | Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | JIANG Xijuan | Combine Traditional Chinese and Western Medicine Institute of Tianjin University of Traditional Chinese Medixine, Tianjin 300193, China | | | FAN Yingchang | Combine Traditional Chinese and Western Medicine Institute of Tianjin University of Traditional Chinese Medixine, Tianjin 300193, China | | | PANG Xiaoli | Nursing School, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | 403033115@qq.com |
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| Abstract
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| [Objiective] To explore the mechanism and effect of rapamycin on plaque in ApoE-/- atherosclerotic mice.[Methods] ApoE-/- mice were divided into three groups:model group, rapamycin group and rosuvastatin group. There groups were fed with high cholesterol lipid feeding about 12 weeks, and then were treated with intragastric administration about 4 weeks. After killing at the end of experiment, the samples of aorta were collected for HE staining and Oil red staining to evaluate lipid nuclear and the area of plaque, using ELISA evaluating the content of TNF-α, INF-γ, IL-6, TGF-β, IL-10 in the serum of peripheral blood.[Results] The area of plaque was measured by image analysis after HE was found that rapamycin group was obviously reduced than model group (P<0.05). In terms of inflammatory factors, content of TNF-α, INF-γ, IL-6 in rapamycin group was marked decreased than model group (P<0.05). However, the level of IL-10,TGF-β was obviously increased comparing with model group (P<0.05).[Conclusion] Rapamycin can shrink the area of plaque and then reduce degree of vascular stenosis, which mechanism may be related to regulate the inflammation response. |
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