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| Study on effect of Tangshen prescription-containing serum on proliferation of renal tubular epithelial cells induced by high glucose and intervention of TGF-β1 and HO-1 |
| Hits 1570 Download times 1213 Received:April 15, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2018.09.15 |
| Key Words
Tangshen prescription;renal tubular epithelial cell;HO-1;TGF-β1 |
| Author Name | Affiliation | E-mail | | ZHAO Xi | Department of Nephropathy, The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China 2. Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | YANG Xikai | | | | WANG Yaoguang | Department of Nephropathy, The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China 2. Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | imanman614@163.com | | LI Man | Beichen Hospital of Traditional Chinese Medicine, Tianjin 300400, China | |
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| Abstract
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| [Objective] To investigate the effect of Tangshen prescription-containing serum on the proliferation of renal tubular epithelial cells induced by high glucose and the intervention of TGF-β1, α-SMA and HO-1.[Methods] Rat serum containing drugs was prepared; cultured renal tubular epithelial cells were divided into blank control group, high glucose group (30 mmol/L glucose), blank serum group (30 mmol/L glucose + 10% blank serum), and sugar kidneys Low-dose group (30 mmol/L glucose + 10% low dose drug-containing serum), high-dose sugar kidney group (30 mmol/L glucose + 10% high-dose drug serum). MTT assay was used to detect the effect of Tangshen prescription-containing serum on proliferation of renal tubular epithelial cells induced by high glucose. The expression of TGF-β1, HO-1 and α-SMA protein in renal tubular epithelial cells was detected by RT-PCR and Western blotting.[Results] Induced by high glucose, renal tubular epithelial cells lost their intrinsic morphology, exhibited a fibroblast-like long spindle shape, and showed a fusiform change in the nucleus. MTT assay:compared with the blank group, the photometric value of the high glucose group was significantly higher (P<0.05); compared with the high glucose group, the absorbance values of the high and low dose drug-containing serum groups decreased after 24 hours of culture, and the difference in absorbance was statistically significant(P<0.05), and the photometric value of high-dose group decreased more than that of low-dose group (P<0.05). The results of Western blotting and RT-PCR showed that:compared with the control group, the levels of TGF-β1 and α-SMA in the high glucose group increased; after intervention with the serum containing the drug, the high glucose induced HK-2 cells TGF-β1 The content of α-SMA decreased, and the difference was statistically significant (P<0.05). Compared with the high-dose and low-dose group of Tangshenfang, the effect of high-dose group was better (P<0.05) in reducing TGF-β1, and α-SMA was decreased. There was no difference in the aspects (P>0.05). The contents of HO-1 was slightly increased after induction of high glucose, compared with the blank control group, the difference was statistically significant (P<0.05); after intervention of Tangshen prescription containing serum, the content was increased, compared with the high glucose group The difference was statistically significant (P<0.05). There was no difference in elevated HO-1 levels between the high-dose group and the low-dose group (P>0.05).[Conclusion] Tangshen prescription can inhibit the deposition of extracellular matrix components, reduce the oxidative stress response, and reduce the cells by inhibiting the proliferation of tubular epithelial cells induced by high glucose and interfering with the expression of TGF-β1, α-SMA, and HO-1 related molecules. In terms of injury, the epithelial-mesenchymal transition was reversed, thereby inhibiting renal interstitial fibrosis and delaying the progression of diabetic nephropathy. |
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