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| Effect of Allitridi on apoptosis and cell cycle of human tongue squamous cell carcinoma Tca8113 cells |
| Hits 1348 Download times 1204 Received:April 02, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2018.09.16 |
| Key Words
Allitridi;tongue squamous cell carcinoma;Tca8113 cell;apoptosis;cell cycle |
| Author Name | Affiliation | | GUO Yanjun | Oral and Maxillofacial Surgery, Cangzhou Central Hospital, Cangzhou 061001, China | | LIU Hongli | Cangzhou Medical College, Cangzhou 061000, China | | CHEN Yong | Oral and Maxillofacial Surgery, Cangzhou Central Hospital, Cangzhou 061001, China | | YAN Wei | Oral and Maxillofacial Surgery, Cangzhou Central Hospital, Cangzhou 061001, China | | WEN Kai | Oral and Maxillofacial Surgery, Cangzhou Central Hospital, Cangzhou 061001, China |
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| Abstract
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| [Objective] To study the effect of Allitridi on apoptosis and cell cycle of human tongue squamous cell carcinoma Tca8113 cells.[Methods] The human tongue squamous cell carcinoma Tca8113 cells in logarithmic growth period were divided randomly into five groups:normal control group, Allitridi (50, 25, 12.5 μg/mL) groups and Cisplatin 40 μg/mL group. 48h after the drugs were given, the cellular growth inhibition rate was detected by MTT, cell cycle and apoptosis rate were analyzed by flow cytometry, the expression of Bax and Bcl-2 mRNA were detected by RT-PCR; the expression of cycle-related regulation of protein (cyclin B1, cyclin D1) and cyclin-dependent kinase (CDK1, CDK2) were detected by Western blotting.[Results] Compared with normal control group. The ratio of the cells in G2/M phase in Allitridi (50, 25 μg/mL) groups were significantly increased (P<0.05), the apoptosis rate were significantly increased (P<0.01); the expression of Bcl-2 mRNA were significantly decreased, while the expression of Bax mRNA were significantly increased and the ratio of Bax/Bcl-2 were significantly increased (P<0.05, P<0.01); the expression of Cyclin B1 protein and CDK1 protein were significantly up-regulated, the expression of cyclin D1 protein and CDK2 protein were significantly down-regulated (P<0.05, P<0.01).[Conclusion] Allitridi can promote human tongue squamous cell carcinoma Tca8113 cells apoptosis and block cell mitosis, which perhaps related to its effects of altering the expression of apoptosis-related regulatory genes and cell cycle-related regulatory proteins. |
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