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| Mechanism study of sulforaphane on cell apoptosis in human gastric cancer cells through Traf6/TAK1 signaling pathway |
| Hits 2216 Download times 1284 Received:May 20, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2018.09.19 |
| Key Words
sulforaphane;human gastric cancer cell;proliferation;apoptosis;mechanism |
| Author Name | Affiliation | E-mail | | ZHU Tao | Department of Gastroenterology, Central Hospital of En-shi Autonomous Prefecture, Enshi 445000, China | | | WANG Yongcui | Enshi Maternal and Child Health Care and Family Planning Service Center, Enshi 445000, China | 89131924@qq.com |
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| Abstract
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| [Objective] To study the impacts of sulforaphane on the apoptosis of human gastric cancer HGC27 cells and its roles in Traf6/TAK1 signaling pathway, and to verify the mechanisms.[Methods] The HGC27 cells were divided into control group, low dose of sulforaphane group (15 μg/mL), middle dose of sulforaphane group (30 μg/mL), and high dose of sulforaphane group (60 μg/mL). After cells were supplemented with varying doses of sulforaphane, the inhibitory rates of cell proliferation were determined using CCK8 assay, the apoptotic rates were assessed using flow cytometry, and the expression levels of Caspase-3, Bax, Bcl-2, Traf6, TAK1 and p-TAK1 were observed by Western Blot.[Results] Compared with control group, the inhibitory rates of proliferation and the apoptotic rates of cells in different sulforaphane doses groups were greatly increased (P<0.05); the expression levels of Caspase-3 and Bax were increased while the level of Bcl-2 was greatly decreased compared with control group (P<0.05); the expression levels of Traf6, p-TAK1 were also greatly increased (P<0.05).[Conclusions] Sulforaphane can suppress the proliferation of HGC27 cells and induce the apoptosis. Traff6/TAK1 signaling pathway may be involved in the inhibitory effect of sulforaphane on the tumor cells. |
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