|
| Establishment and evaluation of phlegm and blood-stasis syndromn on coronary heart disease model by high-fat diet combined with ligation of left anterior descending coronary artery in rats |
| Hits 1619 Download times 1140 Received:July 25, 2018 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2018.10.03 |
| Key Words
coronary heart disease;phlegm and blood stasis syndrome;blood;stasis;phegm animal model;rat |
| Author Name | Affiliation | E-mail | | WANG Shuo | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LIANG Ru | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | XU Huiyu | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | FENG Man | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Pengwei | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | GAO Shuming | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Lin | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | GAO Shan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | PAN Ye | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | phydfr@126.com | | YU Chunquan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | ycq-4@163.com |
|
| Abstract
|
| [Objective] This experiment used high-fat diet feeding combined with ligation of the left anterior descending coronary artery to simulate the pathogenesis of phlegm and blood-stasis syndromn of coronary heart disease (CHD), in order to explore the establishment of rat model of phlegm and blood-stasis syndromn of CHD.[Methods] Healthy SPF Wistar male rats were randomly divided into control group, sham group, phlegm group, blood-stasis group, and phlegm and blood-stasis group. The control group, sham group, and blood-stasis group were fed with normal diet. Both phlegm group and blood-stasis group were fed with high-fat diet. At the end of the 8th week, the left coronary artery ligation were performed on the blood-stasis group and the phlegm and blood-stasis group. The sham group was performed by opening the chest without with ligation. To collect echocardiogram, detect serum lipids, myocardial enzyme index and hemorrheology index, platelet aggregation index in rats serum; meanwhile, to measure and observe HE staining and TTC staining of heart tissue to evaluate the establishment of rat model of phlegm and blood-stasis syndrome of CHD.[Results] Ultrasound changes:compared with the sham operation group, EF, FS, IVSd, IVSs, LVPWS, LV M were significantly decreased, LVIDs and LV Vols were significantly increased (P<0.05, P<0.01) in phlegm and blood stasis syndrome group. Blood lipid changes:compared with the sham operation group, TC and LDL were significantly increased in the phlegm and blood stasis syndrome group, and HDL was significantly decreased (P<0.01). Myocardial enzyme changes:compared with the sham operation group, CK and CM-MB and LDH were significantly increased in the phlegm and blood stasis syndrome group (P<0.01). Hemorheological changes:compared with the sham operation group, BV1/s, BV5/s, BV30/s, BV200/s and PV were significantly increased in the phlegm and blood stasis syndrome group (P<0.05, P<0.01). Platelet aggregation changes:compared with the sham operation group, the PAR and MPAR of the phlegm and blood stasis syndrome group were significantly increased (P<0.01); HE staining, TTC staining:In the phlegm and blood stasis syndrome group, there were breaked and disorderly myocardial fiber, ande the muscle fiber gaps got wider, full of massive swelling mesenchym, inflammatory and necrotic cells.[Conclusion] It suggested the method that is high-fat diet feeding combined with left anterior descending coronary artery ligation can successfully establish the rat model of Phlegm and blood-stasis syndrome of CHD. |
|
|
|
|
|
|
|
