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| Effect of Tangshen No.1 prescription contained serum on the levels of TGF-β1, α-SMA, FN mRNA and protein in HK-2 Cells cultured in high glucose environment |
| Hits 1663 Download times 1133 Received:May 28, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2018.10.13 |
| Key Words
Tangshen No.1 prescription;diabetic nephropathy;HK-2 cells;renal fibrosis;TGF-β1 |
| Author Name | Affiliation | E-mail | | WANG Lijun | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | YANG Xikai | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | XU Bing | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHAO Xi | The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | | | WANG Yaoguang | The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | wangyaoguang1012@126.com |
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| Abstract
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| [Objective] To observe the effect of Tangshen No.1 prescription on the mRNA and protein levels of TGF-β1, α-SMA, and FN in HK-2 cells cultured under high glucose conditions, and preliminary explore the possible mechanism of Tangshen No.1 prescription in the treatment of diabetic nephropathy.[Methods] Renal tubular epithelial cells (HK-2) were selected and divided into blank group, high-sugar group, blank serum group, low-dose group of Tangshen No.1 prescription-containing serum, and high-dose group of Tangshen No.1 prescription-containing serum. Cells were performed in a high glucose environment for 24 hours; serum was intervened for 24 hours. Cells were harvested after 48 hours of co-culture. Use RT-PCR and western blotting methods detected TGF-β1, α-SMA, FN mRNA and protein expression levels in HK-2 cells.[Results] 1) Compared with the blank group, the expression levels of TGF-β1, α-SMA, FN mRNA and protein in the high glucose group increased significantly, the difference was statistically significant (P<0.05). 2) Compared with the high-sugar group, the expression levels of TGF-β1, α-SMA and FN mRNA in the low-dose group of Tangshen No.1 and high-dose group of Tangshen No.1 were significantly decreased, and the difference was statistically significant (P<0.05). 3) Compared with the high-sugar group, the expression of FN protein in the low-dose group of Tangshen No.1 low-dose group was significantly reduced (P<0.05). α-SMA and FN protein in high-dose group of Tangshen No.1. The expression level was significantly reduced and the difference was statistically significant (P<0.05). 4) Compared with the low serum concentration group, the serum TGF-β1 and FN levels in high-concentration sera group decreased more significantly (P<0.05).[Conclusion] The Tangshen No.1 prescription serum significantly inhibited the expression of TGF-β1, α-SMA, FN mRNA and α-SMA and FN protein, and the effect of high concentration of drug-containing serum was superior to that of low-concentration drug-containing serum. |
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