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| Impacts of sulforaphane on proliferation, apoptosis and PI3K/Akt pathway of human colon cancer HT-9 cells |
| Hits 2045 Download times 1385 Received:May 15, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2018.10.15 |
| Key Words
sulforaphane;human colon cancer cells;proliferation;apoptosis;mechanism |
| Author Name | Affiliation | E-mail | | ZHU Tao | Department of Gastroenterology, Central Hospital of Enshi Autonomous Prefecture, Enshi 445000, China | | | ZHANG Jigui | Endoscopy Center, Central Hospital of Enshi Autonomous Prefecture, Ensh 445000, China | 303715495@qq.com |
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| Abstract
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| [Objective] To observe the impacts of sulforaphane on proliferation, apoptosis and PI3K/Akt pathway of human colon cancer HT-9 cells.[Methods] The effects of sulforaphane on proliferation of HT-9 cells were investigated using CCK8 assays. The HT-9 cells apoptosis was analyzed by flow cytometry with propidium iodide staining. The expression levels of related proteins in PI3K/Akt pathway and apoptosis proteins were analyzed by Western Blot.[Results] Sulforaphane can significantly suppress proliferation of HT-9 cells in a time-and dose-dependent manner. Propidium iodide staining showed that the apoptosis of HT-9 cells was stimulated by sulforaphane. After treated with 10, 20, 40 μg/mL of sulforaphane for 24 h, the apoptosis rates were (14.67±1.95)%, (27.95±2.53)% and (35.6±3.75)%, respectively. Sulforaphane downregulated the expression levels of PI3K, p-Akt and Bax proteins and upregulated the expression of Bax protein in a dose-dependent manner.[Conclusion] Sulforaphane can significantly suppress the proliferation of HT-9 cells and stimulate apoptosis, the mechanism may be associated with the inhibition of PI3K/Akt signaling pathway. |
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