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| Screening of composition of Chanwucao Fraction powder and its pharmacodynamic effects in vitro |
| Hits 1928 Download times 1212 Received:June 22, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2018.10.18 |
| Key Words
cinobufagin;schisandra chinensis;astragalus;glycyrrhizic acid;herbalome;tumor |
| Author Name | Affiliation | | CHEN Guibing | Center of General Surgery, The People's Liberation Army General Hospital of Chengdu Command, Chengdu 610083, China |
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| Abstract
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| [Objective] To screen compatible herbs of efficacy enhancing and toxicity reducing on Cinobufagin fraction of the skin of Bufo bufo gargarizans Cantor (CFSB) and observe its activity influence to tumorous and normal cell lines in vitro.[Methods] To optimize compatibility about CFSB, Schisandra chinensis fraction, Astragalus fraction and Glycyrrhizic acid by applying Factorial design (24) and Orthogonal design L9(34). Then, to detect the survival rate of SW620, L02, CIK with this Prescription interfered by CCK-8 method in different time. Last, to detect release of the ALT and AST of L02 cell under this Prescription interfered.[Results] Factorial design has determined that four Chinese herbs have the interaction and this Prescription consists of the four Chinese herbs. Further Orthogonal design optimizes the compatibility doses, which is CFSB 2 μg/mL, Schisandra chinensis fraction 10 μg/mL, Astragalus fraction 5 μg/mL and Glycyrrhizic acid 10 μg/mL. This Prescription which is completed combination is named Chanwucao Fraction Powder (CFP). To compare with CFSB, CFP can reduce the survival rate of SW620 and increase the survival rate of L02 and CIK. In addition, it can reduce that L02 release ALT.[Conclusion] CFP which is appropriate combination realizes efficacy enhancing and toxicity reducing on CFSB. |
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