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| Establishment and evaluation of tumor necrosis factor-α induced insulin resistance in 3T3-L1 adipocytes |
| Hits 1545 Download times 1084 Received:July 02, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2018.11.14 |
| Key Words
tumor necrosis factor-α;adipocytes;insulin resistance;glucose transporter 4;high content screening |
| Author Name | Affiliation | E-mail | | LIU Lu | Department of Biochemistry, College of Integrated Traditional Chinese and Western Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin Key Laboratory of Modern Traditional Chinese Medicine, Tianjin 300193, China | | | CAO Shijie | Tianjin Key Laboratory of Modern Traditional Chinese Medicine, Tianjin 300193, China | | | CHENG Lina | Department of Biochemistry, College of Integrated Traditional Chinese and Western Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin Key Laboratory of Modern Traditional Chinese Medicine, Tianjin 300193, China | | | QIU Feng | Tianjin Key Laboratory of Modern Traditional Chinese Medicine, Tianjin 300193, China
Department of Chinese Medicinal Chemistry, College of Traditional Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | fengqiu20070118@163.com | | KANG Ning | Department of Biochemistry, College of Integrated Traditional Chinese and Western Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin Key Laboratory of Modern Traditional Chinese Medicine, Tianjin 300193, China | kangndd@163.com |
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| Abstract
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| [Objective] To determine the optimal time and dose of tumor necrosis factor-α (TNF-α)-induced insulin resistance model and to investigate the effect of TNF-α on the translocation of glucose transporter 4 (GLUT4) influences to explore the evaluation of the establishment of the model.[Methods] The insulin resistance model was established by using TNF-α at different concentrations (5, 10, 15, 20, 25 ng/mL) for different time (48, 72, 96 h) in 3T3-L1 adipocytes. In addition, the expression and distribution of GLUT4 protein were detected by Western blot and high content screening (HCS).[Results] The results showed that the insulin resistance model 3T3-L1 cells were established by treating with 10 ng/mL TNF-α for 96 h. Compared with the control group, the glucose uptake and GLUT4 expression were significantly decreased in the model group. After adding insulin, glucose uptake and GLUT4 expression of control group were significantly increased. Furthermore, the translocation of GLUT4 to the plasma membrane was decreased in the insulin resistance model, differences were statistically significant.[Conclusion] TNF-α can significantly inhibits the uptake of glucose and the translocation of GLUT4 to the plasma membrane in insulin resistance model induced by treating with 10 ng/mL TNF-α for 96 h in 3T3-L1 adipocytes, and therefore supplemented the insulin resistance model established by the evaluation criteria. |
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