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| Inhibitory effect of oxymatrine on apoptosis in hippocampal CA1 region of the rats with chronic cerebral ischemia |
| Hits 1485 Download times 1034 Received:June 06, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2018.11.17 |
| Key Words
oxymatrine;cerebral ischemic;hippocampal CA1 region;apoptosis;oxidative stress |
| Author Name | Affiliation | | YU Ting | Department of Internal Medicine, Handan Traditional Chinese Medicine, Handan 056001, China | | HAN Yafei | Fourth Department of Neurosurgery, Handan Central Hospital, Handan 056001, China |
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| Abstract
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| [Objective] To study the inhibitory effect of oxymatrine on apoptosis in hippocampal CA1 region of the rats with chronic cerebral ischemia.[Methods] The chronic cerebral ischemia model was established by ligating bilateral common carotid artery, setting sham operating group, model group and oxymatrine low, medium, high-dose (25, 50, 100 mg/kg) groups, n=20. The drugs were given by intraperitoneal injection from the second day after operation for 7 d. The morphological changes of neurons in hippocampus CA1 region were observed by HE Staining, the cell apoptosis were observed after TUNEL staining; the expression of Bcl-2 and Bax were exzamed by IHC, the expression of Caspase-3, NF-κB were determined by Western blotting, the activity of antioxidant enzymes and malondialdehyde (MDA) in Hippocampus tissue were measured by biochemical analysis.[Results] Compared with model group, both of the hippocampal CA1 neuronal pathological changes and apoptosis were significantly improved, the apoptosis index (AI) were significantly decreased (P<0.01). The expression of Bcl-2 were significantly up-regulated and the expression of Bax were down-regulated, increased the Bcl-2/Bax ratio (P<0.05 or P<0.01). The activity of caspase-3 and NF-κB were significantly decreased (P<0.05 or P<0.01). The activity of antioxidant enzymes (SOD, CAT) activity were significantly improved and the content of MDA were significantly decreased (P<0.05 or P<0.01).[Conclusion] Oxymatrine has a pharmacological effect of inhibiting apoptosis of hippocampal CA1 region in rats with chronic cerebral ischemia, which may be related to the regulation of oxymatrine apoptosis-related protein expression and reduce oxidative stress damage. |
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