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| Protective effects of puerarin on oxidative stress in BV-2 microglia through Nrf2/ARE signaling pathway |
| Hits 1491 Download times 1070 Received:August 20, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2018.12.19 |
| Key Words
puerarin;microglia;oxidative stress |
| Author Name | Affiliation | E-mail | | LI Bing | Department of Pharmacy, Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China | | | ZHANG Chan | Department of Pharmacy, Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China | | | LIN Fang | Department of Pharmacy, Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China | | | LI Ziming | Department of Pharmacy, Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China | | | ZHOU Jian | Department of Pharmacy, Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China | vulgar78@sohu.com |
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| Abstract
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| [Objective] To investigate the protective effects of puerarin on BV-2 microglia injury stimulated by H2O2, and investigate its possible mechanism.[Methods] BV-2 cells were cultured in vitro and H2O2 was used to induce the oxidative stress model of cells. The cells were divided into five groups:blank control group, H2O2 induced model group, H2O2 plus puerarin treatment groups (5, 10, 20 μg/mL). CCK-8 kit was used to measure the relative survival rate of BV-2 cells and related kits were used to analyze the levels of MDA, NO and the activity of SOD. Western Blot was used to determine the expression levels of Nrf2, GCLC, NQO1 and HO-1.[Results] H2O2 can induce oxidative stress injury on BV-2 cells. The activity of SOD after treatment with different concentrations of puerarin were significantly increased compared to H2O2 induced model group, while the expression levels of MDA and NO were significnatly decreased compared with model group. The results of Western Blot indicated that the Nrf2 translocation was significantly increased after treatment for puerarin, and the levels of GCLC, NQO1 and HO-1 were greatly increased (P<0.05).[Conclusion] Puerarin can significantly inhibit the oxidative stress injury of BV-2 cells and its underlying mechanism may involve in activating the activation of Nrf2/ARE signaling pathway. |
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