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| Effect of Xinnao Shutong Capsules on secretion of neurotrophic factor in oxygen-glucose deprivation/reoxyge nation astrocytes and protection of neurons |
| Hits 2096 Download times 1549 Received:August 28, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2019.01.17 |
| Key Words
Xinnao Shutong Capsules;astrocyte;neuron;brain derived neurotrophic factor;glial cell-derived neurotrophic factor |
| Author Name | Affiliation | E-mail | | LI Ruilin | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LIU Wenjie | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | YUAN Qing | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHANG Tong | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | XU Yao | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HU Limin | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae Ministry of Education, Tianjin 300193, China | | | CHAI Lijuan | Tianjin Key Laboratory of Chinese Medicine Pharmacology, Tianjin 300193, China | cljuan1258@163.com |
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| Abstract
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| [Objective] To explore the effect of Xinnao Shutong Capsules on the secretion of nerve growth factor in astrocytes cell induced by oxygen-glucose deprivation/reoxygenation (OGD/R). The protective effects on OGD/R-induced neurons cell variability treated by astrocytes conditioned medium were also explored.[Methods] The effect of Xinnao Shutong Capsules on both normal culture and OGD/R-induced C8-D1A cell viability were detected by CCK-8. ELISA was adopted to measure the secretion of BDNF, GDNF and CNTF in OGD/R-induced C8-D1A cell which treatment with Xinnao Shutong Capsules.[Results] 1) Xinnao Shutong Capsules had no significant effect on the viability of normal cultured C8-D1A cell, but could increase the proliferation of OGD/R-induced C8-D1A cell. 2) Xinnao Shutong Capsules (0.1 μg/mL) could significantly promote the secretion of BDNF on C8-D1A cell after OGD/R. 3) C8-D1A cell treated with Xinnao Shutong Capsules (0.1 μg/mL) conditioned medium had protective effect on neurons.[Conclusion] Xinnao Shutong Capsules had protective effect on OGD/R-induced C8-D1A cell, and its treated C8-D1A conditioned medium played protective role on OGD/R-induced neurons. |
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