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| Protective effect and mechanism of Silibinin on isolated heart tissue ischemia-reperfusion injury |
| Hits 2056 Download times 1347 Received:September 30, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2019.01.18 |
| Key Words
Silibinin;Langendorff perfusion;isolated heart;hemodynamics;ATPase |
| Author Name | Affiliation | | CAO Xudan | Handan Central Hospital, Handan 056001, China | | REN Chunmei | Handan Central Hospital, Handan 056001, China | | HE Tiantian | Handan Central Hospital, Handan 056001, China | | LIi Suhua | Handan Central Hospital, Handan 056001, China | | LI Li | Handan City Infectious Disease Hospital, Handan 056001, China |
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| Abstract
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| [Objective] To study the protective effect of Silybin (SIL) on Langendorff perfusion rat isolated from ischemia-reperfusion injury and study its mechanism.[Methods] 100 SD rats were randomly divided into normal control group, model group and SIL low-dose, medium-dose, high-dose[100, 200, 400 mg/(kg·d)], n=20. Two hours before surgery, the drugs were given by intragastric administration. The heart perfusion model was established by Langendorff perfusion system, the normal control group continued infusion of K-H solution, model group and salvia miltiorrhiza polyphenolic acid group were perfused 20 min, and then subjected ischemia for 30 min, followed by a 60 min reperfusion period. The myocardial enzyme activity in coronary effluent were determined; TTC staining was used to measure the myocardial infarct size, the cardiac tissue lesions were observed by HE staining; the hemodynamic parameters were determined; the activities of antioxidant enzymes and malondialdehyde (MDA) in myocardium were measured; the activity of Na+-ATPase and Ca2+-ATPase in myocardium was measured.[Results] Compared with model group, the activity of AST, LDH and CK-MB of SIL medium-dose, high-dose were significantly increased; the cardiac tissue infarct size were significantly reduced, cardiac lesions were significantly improved; the hemodynamic parameters were significant improved:the HR, LVSP, +dP/dtmax, -dP/dtmin were significantly increased and LVEDP was significantly decreased; the activity of SOD, CAT in myocardium were significantly increased and the content of MDA were significantly decreased; the activity of Na+-ATPase, Ca2+-ATPase were significantly increased; all of the difference above were significant (P<0.05 or P<0.01).[Conclusion] The SIL has a certain inhibitory effect on isolated heart ischemia-reperfusion injury, which perhaps related to its effects of improving hemorheology, reducing oxidative stress injury and improving ATPase activity. |
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