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| Effect of Jiaotai Pills and its disassembled prescription on ICa-L in single guinea-pig ventricular myocytes |
| Hits 1362 Download times 1127 Received:August 20, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2019.01.19 |
| Key Words
Jiaotai Pill;Rhizoma Coptidis;Cortex Cinnamomi Cassiae;ICa-L;serum pharmacology;kinetic parameter |
| Author Name | Affiliation | E-mail | | XING Zuoying | Heart Centre, The First Affiliated Hospital of Henan University, Zhengzhou 450000, China | | | WANG Yongxia | Heart Centre, The First Affiliated Hospital of Henan University, Zhengzhou 450000, China | wyxchzhq@163.com | | ZHU Mingjun | Heart Centre, The First Affiliated Hospital of Henan University, Zhengzhou 450000, China | | | CHEN Zhaoqi | Publicity Department, Henan Provincial People's Hospital, Zhengzhou 450000, China | | | SONG Huanhuan | Heart Centre, The First Affiliated Hospital of Henan University, Zhengzhou 450000, China | | | GAO Yuan | Heart Centre, The First Affiliated Hospital of Henan University, Zhengzhou 450000, China | | | HU Yucai | Heart Centre, The First Affiliated Hospital of Henan University, Zhengzhou 450000, China | | | CHEN Peng | Heart Centre, The First Affiliated Hospital of Henan University, Zhengzhou 450000, China | | | ZHENG Jia | Heart Centre, The First Affiliated Hospital of Henan University, Zhengzhou 450000, China | |
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| Abstract
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| [Objective] To explore the effect of Jiaotai Pills and its disassembled prescription in drug serum on ICa-L in guinea-pig ventricular myocytes.[Methods] Acute isolated guinea pig ventricular muscle cells and explore the effect of drug on ICa-L current and and channel dynamics parameters. The myocytes were randomly divided into five groups:the control group, the Rhizoma Coptidis group (decoction of 100 g goldthread), the Cortex Cinnamomi Cassiae group (decoction of 10 g cinnamon), the Rhizoma Coptidis and Cortex Cinnamomi Cassiae group(single decoction of 100 g goldthread + equal volume decoction of 10 g cinnamon), the Jiaotai Pills group (co-decoction of 100 g goldthread and 10 g cinnamon). Cells were divided into 10% and 15% drug concentration groups and incubated at 37℃ in ahumidified, 5% CO2 incubator after 24 h, patch clamp test was performed.[Results] Compared with the control group, the drug groups (10%, 15%) inhibited ICa-L density to different degrees (P<0.05), and the highest inhibition rate of 10% concentration Jiaotai Pills was 57.5%. All groups made the activation, inactivation and recovery time contant prolonged (P<0.05). The rate of activation and inactivation time constant of Cortex Cinnamomi Cassiae group was the largest, and the rate of recovery time constant of Rhizoma Coptidis group was the largest.[Conclusion] Drug containing serum of Jiaotai Pills and disassembled prescription have different degrees of inhibition on calcium ion channels, and the mechanism of action are same with calcium blockers, it could be the mechanism of Jiaotai Pills antiarrhythmic. |
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