|
| Optimization of extraction process of walnut meal protein and activity of enzymatic hydrolysate |
| Hits 1778 Download times 1179 Received:October 18, 2018 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2019.01.23 |
| Key Words
Walnut meal protein;extraction process;optimization;enzymatic hydrolysate;activity |
| Author Name | Affiliation | E-mail | | XUE Nannan | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China | | | FANG Kang | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China | | | ZHANG Yuehui | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China | | | CHEN Meng | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China | | | WANG Hui | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China | | | ZHANG Xinyu | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China | | | WU Gaorong | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China | | | Guo Wenbo | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China | | | JIA Xiaohui | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China | | | WANG Penglong | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China | wpl581@126.com | | LEI Haimin | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China | hm_lei@126.com |
|
| Abstract
|
| [Objective] Optimize the extraction process of walnut meal protein by response surface methodology(RSM), and evaluate its antioxidant activity of papain hydrolysate, which provided a reference for the subsequent development and utilization of walnut meal protein.[Methods] The alkali-soluble acid precipitation method was used to optimize the parameters (NaOH concentration, liquid-to-material ratio, extraction temperature and time) of extraction process with the result of single factor and RSM experimentation. The papain hydrolysate was tested for its antioxidant activity.[Results] The result of RSM was as follows:80:1 liquid-to-material ratio, 0.04% NaOH solution, 65℃ for 1.0 h, with 35.27% of extraction rate. The extraction rate of three parallel experiments was up to 36.01% closed to the theoretical value, indicating that the process is real and reliable, convenient and feasible; papain hydrolysate of walnut protein effected on scavenging DPPH, OH radical and reducing activity, which could provide basis for the subsequent development and industrial production of walnut health food.[Conclusion] This paper successfully optimized the extraction process of walnut meal protein by RSM, and provided an important reference value for the comprehensive development and utilization of walnut meals. |
|
|
|
|
|
|
|
