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| Effect of Maimendong Decoction on bone marrow mesenchymal stem cells(BMSC) to differentiate into alveolar epithelial cells of rat |
| Hits 1404 Download times 1057 Received:November 16, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2019.02.20 |
| Key Words
Maimendong Decoction;bone marrow mesenchymal stem cells;pulmonary fibrosi;stem cells differentiation |
| Author Name | Affiliation | E-mail | | LIU Rui | Department of Geriatrics, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530011, China | | | LEI Ningning | Guangxi University of Chinese Medicine, Nanning 530011, China | | | WU Juanjuan | Department of Geriatrics, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530011, China | | | YE Chuandong | Guangxi University of Chinese Medicine, Nanning 530011, China | | | CHEN Sining | Department of Geriatrics, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530011, China | chensining2007@163.com |
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| Abstract
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| [Objective] To observe the effect of Maimendong Decoction on bone marrow mesenchymal stem cells(BMSC) to differentiate into alveolar epithelial cells of rats.[Methods] Maimendong Decoction rat serum, rat model of pulmonary fibrosis in rats serum and rat model of pulmonary fibrosis+Maimendong Decoction serum were given on GFP-BMSC. After 14 days, to detect the expression of SP-C, AQP-5 protein and mRNA in GFP-BMSC cells by immunohistochemistry and qPCR method.[Results] The expression of SP-C and AQP-5 proteins in the model group, model+Maimendong Decoction group was significantly higher than that of DMEM control group, Maimendong Decoction group (P<0.01); while the model+Maimendong Decoction group SP-C, AQP-5 proteins expression was significantly higher than the model group (P<0.01).[Conclusion] Maimendong Decoction can promote GFP-BMSC cells into alveolar epithelial cells in pulmonary fibrosis, which may be one of the important mechanisms to improve pulmonary fibrosis. |
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