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| Effect of Polygonatum kingianum on inflammatory factors and oxygen radical of acute lung injury model rat |
| Hits 1430 Download times 986 Received:November 08, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2019.02.22 |
| Key Words
Polygonatum kingianum;acute lung injury rat;inflammatory factors;oxygen radicals |
| Author Name | Affiliation | | HUANG Fengyu | Guigang City People's Hospital, Guigang 537100, China |
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| Abstract
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| [Objective] To investigate the effect of Polygonatum kingianum on inflammatory factors and oxygen radical of acute lung injury model rats.[Methods] The 50 SD male rats were randomly divided into 5 groups, normal control group (NCG), normal model group(NMG), low level group(LLG), middle level group(MLG) and high level group(HLG). Acute lung injury (ALI) model rats were made by intratracheal drip irrigating LPS (5 mg/kg). The Polygonatum kingianum water extract was injected into rats' enterocoelia 4 hours after modeling. 24 hours later, arterial blood was collected and PaO2, amounts of inflammatory cells in alveolar lavage fluid, levels of MOD, MPO, GSH-Px and SOD were measured. ELISA was introduced to test the level of TNF-α, IL-10, IL-1β and IL-6. The LI,W/D and (W-D)/W were tested to value the biological function of rats' lung.[Results] Compared with the NCG, NMG group showed lower levels of LI,W/D and (W-D)/W (P<0.01), and the level in experimental group injected Polygonatum kingianum was higher than NMG (P<0.01). The level of PaO2 was on the contrary, and the trend had dose dependence. The inflammatory cells in NMG group were more than NCG(P<0.01), which in each experimental group were lower than NMG (P<0.05), the number in HLG was higher than MLG obviously (P<0.05). The results of inflammatory factors showed the IL-1β, IL-6 and TGF-α level in NMG was higher than NCG, but IL-10 was lower (P<0.05). The IL-1β, IL-6 and TGF-α level in each experimental group was higher than NMG and IL-10 was lower (P<0.05). The difference was significant between HLG and MLG (P<0.01). MDA and MPO levels in alveolar lavage fluid of the NMG were significantly higher than those of the NCG, and lower activity of SOD and GSH-Ps (P<0.05). The level of MDA, MPO of experimental groups were lower than NMG, activity of SOD and GSH-Px were higher than NMG (P<0.01). The difference was significant between HLG and MLG(P<0.05).[Conclusion] Chinese medicine of Polygonatum kingianum can relief symptoms of thoracic injury induced by LPS, optimizing inflammation levels, reducing oxygen free radical injury of ALI rats' lung tissue, and the dose-dependence is obvious. |
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