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| Effect of Neiyi Zhitong Prescription for relieving pain on E-cadherin and β-catenin gene transcription in EMs rat |
| Hits 1994 Download times 1029 Received:September 21, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2019.02.23 |
| Key Words
endometriosis;adhesion;β-catenin;E-cadherin;Neiyi Zhitong Prescription |
| Author Name | Affiliation | E-mail | | ZHU Xiaolin | The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150001, China | | | HAN Yaguang | The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150040, China | 13945053676@126.com | | BAO Lei | The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150040, China | | | GUO Wenhai | The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150001, China | | | ZHANG Xuezhi | The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150040, China | | | WANG Xuelian | The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150040, China | | | HAN Yanhua | The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150040, China | |
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| Abstract
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| [Objective] The expression of β-catenin and E-cadherin gene in ectopic focus tissue of rats with EMs was detected by RT-PCR technique by constructing rat model of endometriosis. To investigate the therapeutic effect of Neiyi Zhitong Prescription on β-catenin and E-cadherin.[Methods] The 57 SD rats were selected and EMs model was constructed by autologous transplantation. The success of the model construction was observed by laparotomy 4 weeks later,and 5 model rats were selected for histopathological evaluation by HE staining. The 40 EMs rats successfully modeled were randomly divided into 4 groups,model group,high-dose and low-dose Chinese medicine treatment group,and positive drug group,with 10 rats in each group and 8 rats in the sham operation group (open resection only,no autologous transplantation). In the high-dose and low-dose groups of traditional Chinese medicine,985.3 mg/(kg·d) and 245.8 mg/(kg·d) by Neiyi Zhitong prescription were given. The positive drug group was given 7.2 mg/(kg·d) danazole by gavage,and the model group and the sham operation group were given 2 mL/(kg·d) normal saline by gavage. Each group was given the drug once a day for 4 weeks. Transcription levels of β-catenin and E-cadherin in ectopic lesion tissues of EMs model rats were detected by RT-PCR.[Results] Compared with the model group,Neiyi Zhitong Prescription can significantly increase the mRNA of E-cadherin and reduce the transcription level of β-catenin mRNA in EMs model rats,and the transcription level of each gene in the high-dose group of traditional Chinese medicine is significantly better than that in the low-dose group (P<0.01).[Conclusion] Neiyi Zhitong Prescription can maintain the structural and functional stability of the E-cad/β-cat complex by up-regulating the transcription of E-cadherin or down-regulating the transcription of β-catenin gene,thus enhancing the adhesion between cells in the endometrium. |
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