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| Experimental research of quercetin inhibits proliferation and migration of human cervical cancer cells via inhibiting β-catenin |
| Hits 2214 Download times 1362 Received:September 28, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2019.02.25 |
| Key Words
quercetin;cervical cancer cell;Wnt/β-catenin signaling pathway;cell proliferation;cell migration |
| Author Name | Affiliation | | WANG Xiangqing | Department of Gynaecology and Obstetrics, 464 Hospital of the PLA, Tianjin 300381, China | | MA Zhenjun | Department of Gynaecology and Obstetrics, 464 Hospital of the PLA, Tianjin 300381, China | | BAO Hongyun | Department of Gynaecology and Obstetrics, 464 Hospital of the PLA, Tianjin 300381, China | | WANG Ru | Department of Gynaecology and Obstetrics, 464 Hospital of the PLA, Tianjin 300381, China | | CHEN Xuelian | Department of Gynaecology and Obstetrics, 464 Hospital of the PLA, Tianjin 300381, China | | LIU Zhiyong | Department of Pathology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China | | KONG Fangfang | Department of Gynaecology and Obstetrics, 464 Hospital of the PLA, Tianjin 300381, China |
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| Abstract
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| [Objective] To investigate the effects of quercetinon proliferation and migration of human cervical cancer (CC) cells by Wnt/β-catenin signaling pathway and its mechanism and to provide a new gene target in the treatment of CC.[Methods] The research objects were the human CC HeLa cells. After treatment with different concentrations ofquercetin for 24 hours, the proliferation of HeLa cells was detected by CCK-8 test, the horizontal and vertical migration were detected by Transwell and wound scraching assays, the activation of Wnt/β-catenin signaling pathway was detected by β-catenin immunofluorescence assay and TOP-flash luciferase reporter system.[Results] CCK-8 test indicated that the cell proliferation rate of the quercetin group was significantly lower than the control group (P<0.01), and the inhibition of quercetinon proliferation was dose-dependent. The horizontal and vertical migration of Hela cells in the quercetin group was significantly decreased compared to the control group(P<0.01). The β-catenin immunofluorescence was distributed in nuclear and cytoplasm in the control cells, while mainly in the cytoplasm in the quercetin-treated cells. Top Flashluciferase reporter system indicated the Wnt/β-catenin signaling pathway was inhibited in the quercetin-treated cells.[Conclusion] Quercetin may inhibit proliferation and migration of human cervical cancer cells via inhibiting Wnt/β-catenin signaling pathway. |
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