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| Effects of Xifeng Capsule on the expression of voltage-gated type I sodium channel α subunit protein (Nav1.1) in hippocampus of epileptic rats induced by lithium chloride and pilocarpine |
| Hits 1569 Download times 1315 Received:December 15, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2019.03.17 |
| Key Words
voltage-gated sodium channel α subunit protein;Xifeng Capsule;epilepsy;pilocarpine |
| Author Name | Affiliation | E-mail | | LU Yanli | Paediatrics, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | FANG Yanyan | Paediatrics, Chinese Medicine Hospita in Linyi, Linyi 276002, China | | | LI Xinmin | Paediatrics, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | tjtcmlxm@163.com | | SUN Dan | Paediatrics, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | JIN Li | Paediatrics, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HAN Yaowei | Paediatrics, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | |
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| Abstract
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| [Objective] To study the effect of Xifeng Capsule on the expression of voltage-gated type I sodium channel α subunit protein (Nav1.1) in hippocampus of epileptic rats induced by lithium chloride and pilocarpine.[Methods] Established the epileptic rat model was induced by lithium chloride and pilocarpine. The experimental rats were randomly divided into 5 groups:normal control group (blank group),model control group (model group),low dose group of Xifeng Capsule (low dose group),medium dose group of Xifeng Capsule (medium dose group),and high dose group of Xifeng Capsule (high dose group). The expressions of Nav1.1 in hippocampus of experimental rats were detected by immunohistochemical staining.[Results] Through the expression of Nav1.1,we found the neuronal structure of CA1 and DG regions of epileptic rats were basically normal,and the changes of Nav1.1 were not obvious. In the CA3 area,the neurons in the model group were denatured and necrosis,in which,the staining of Nav1.1 became shallower or even disappeared,and increased in the normal tissues around the denatured and necrotic neurons. In the model group,the degeneration of neurons in the CA3 area was observed,and the staining of Nav1.1 in necrotic site became shallower or even disappeared;while in the treatment group of Xifeng Capsule,the number of degeneration and disappearance of neurons was decreased with the increase of the dose of Xifeng Capsule. At the same time,the reduction in the degree of brown area lessen was positively correlated with the dosage. However,the staining intensity was not obvious in the normal tissues around the denatured and necrotic neurons. Furthermore,from quantitative analysis,the expressions of Nav1.1 number in the model group were increased,while the number did not increase significantly in each Xifeng Capsule treatment group.[Conclusion] Xifeng Capsule may protect hippocampal neurons and regulate the expression of Nav1.1 in hippocampal neurons of IE rats. It is speculated that Xifeng Capsule can play an antiepileptic effect by inhibiting sodium channel and reducing the excitability of neuronal cell membrane. |
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