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| Xingnaojing injection components promote glutamate clearance in hypoxia/reoxygenation astrocytes and improve cell viability in hypoxia neurons |
| Hits 2324 Download times 1714 Received:November 15, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2019.03.19 |
| Key Words
Xingnaojing injection components;astrocytes;glutamate clearance;Hypo/Reox;neuronal viability |
| Author Name | Affiliation | E-mail | | CHAI Lijuan | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin 301617, China
Tianjin Key Laboratory of Chinese Medicine Pharmacology, Tianjin 301617, China
Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | XU Yao | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin 301617, China | | | HUANG Juyang | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin 301617, China
Tianjin Key Laboratory of Chinese Medicine Pharmacology, Tianjin 301617, China | | | LI Ruilin | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin 301617, China | | | GUO Hong | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin 301617, China
Tianjin Key Laboratory of Chinese Medicine Pharmacology, Tianjin 301617, China
Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Shaoxia | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin 301617, China
Tianjin Key Laboratory of Chinese Medicine Pharmacology, Tianjin 301617, China | | | HU Limin | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin 301617, China
Tianjin Key Laboratory of Chinese Medicine Pharmacology, Tianjin 301617, China
Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | huliminth@126.com |
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| Abstract
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| [Objective] To study the effects of eight active components of Xingnaojing injection on glutamate transport in hypoxia/reoxygenation (Hypo/Reox) astrocytes in vitro and astrocyte-conditioned medium on the viability of Hypo/Reox neurons.[Methods] Astrocytes were cultured in vitro and identified by GFAP. Dibutyryl cyclic adenosine monophosphate (dBcAMP) and Hypo/Reox were used to induce astrocytes activation,cell viability was determined by CCK-8. Extracellular glutamate concentrations were measured by the Glutamate Kit. Neurons were cultured with astrocytes conditioned medium,and neuronal cell viability was determined by CCK-8.[Results] Astrocytes express the marker protein GFAP. dBcAMP 0.0625 mmol/L synergistic hypoxia 4 h plus reoxygenation 3 h (Hypo 4 h/Reox 3 h) can significantly reduce the ability of astrocytes to clear extracellular glutamate. The effective components of β-elemene,genistein,camphol and borneol can significantly increase the clearance ability of astrocytes in Hypo 4 h/Reox 3 h. At the same time,they also significantly improve neuronal viability cultured with astrocytes-conditioned medium after Hypo 4 h/Reox 3 h.[Conclusion] XNJI components promote glutamate clearance in hypoxia/reoxygenation astrocytes and improve cell viability in hypoxia neurons. |
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