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| Study on loroglossin induced myeloid derived suppressor cells apoptosis via activating the Bcl-2 signaling pathway |
| Hits 1332 Download times 1067 Received:December 10, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2019.03.20 |
| Key Words
loroglossin;anti-tumor;myeloid derived suppressor cells;apoptosis |
| Author Name | Affiliation | E-mail | | QIN Yue | Key Laboratory of Ethnomedicine, Minzu University of China, Beijing 100081, China | | | PANG Zongran | Key Laboratory of Ethnomedicine, Minzu University of China, Beijing 100081, China | zrpang@163.com |
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| Abstract
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| [Objective] Promoting the apoptosis of myeloid derived suppressor cells is an effective way to inhibit tumor growth. Therefore,this experiment aimed to explore the inhibitory effect and mechanism of loroglossin extracted from Spiranthes sinensis (Pers.) Ames[Neottia sinensis Pers.] on MDSCs.[Methods] MTT assay was used to detect the toxicity of loroglossin to MDSC cell lines. Pro-apoptotic effect of loroglossin on MSC-2 was determined by flow analysis. Western blot was used to detect the protein expression levels of apoptosis-related proteins in MSC-2 cells.[Results] Loroglossin treatment resulted in a dose-dependent decrease in cell proliferation and induced apoptosis in MDSCs. Subsequent analysis showed that loroglossin treatment enhanced activation of JNK2,cytochrome C,caspase-9 and caspase-3,and downregulated the expression levels of Bcl-2.[Conclusion] Loroglossin could promote the apoptosis of MDSCs and enhance the positive immune regulation through activating the Bcl-2 signaling pathway. Loroglossin is promising as a new drug for clinical treatment of cancer. |
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