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| Effects of oxymatrine on hemorheology after focal cerebral ischemia-reperfusion in rats and its mechanism |
| Hits 1451 Download times 1039 Received:November 12, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2019.03.21 |
| Key Words
oxymatrine;cerebral ischemia-reperfusion;hemorheology;nerve function;cell membrane fluidity |
| Author Name | Affiliation | | QU Juanjuan | Department of Emergency, Handan Central Hospital, Handan 056000, China | | WANG Shuangyu | Department of No.1 Nephrology, Handan Central Hospital, Handan 056001, China | | ZHANG Chao | Department of Emergency, Handan Central Hospital, Handan 056000, China | | XU Haifa | Department of Emergency, Handan Central Hospital, Handan 056000, China | | CHEN Huimin | Department of Emergency, Handan Central Hospital, Handan 056000, China | | ZHOU Shaoying | Department of Emergency, Handan Central Hospital, Handan 056000, China | | HAN Yafei | Department of Emergency, Handan Central Hospital, Handan 056000, China | | WANG Chaozong | Department of Emergency, Handan Central Hospital, Handan 056000, China | | YU Ting | Handan Traditional Chinese Medicine, Handan 056001, China |
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| Abstract
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| [Objective] To study the effect of oxymatrine on hemorheology after acute cerebral ischemia-reperfusion in rats and to explore its mechanism.[Methods] The models of focal cerebral ischemic reperfusion were established with Zea-Longa occluding suture,setting sham operating group,model group and oxymatrine low,medium,high-dose (25,50,100 mg/kg) groups,n=20. The drugs were given by intraperitoneal injection from the second day after operation for 7 d,once a day. The neurological deficits,ratio of infarct volume,water content of the brain were evaluated;the brain tissue lesions was observed through HE staining;the activity of antioxidant enzymes and malondialdehyde (MDA) in Hippocampus tissue were measured by biochemical analysis;the hemorheology index and erythrocyte membrane fluidity index were determinend.[Results] Compared with model group,the neurological scores of oxymatrine medium,high-dose groups were significantly decreased (P<0.05 or P<0.01). The ratio of infarct volume and brain water content were significantly decreased (P<0.05 or P<0.01). Brain tissue lesions were significantly improved;reduced blood rheology (whole blood viscosity,plasma viscosity,erythrocyte aggregation index,erythrocyte degeneration index,erythrocyte rigidity index,hematocrit,erythrocyte sedimentation rate,P<0.05 or P<0.01),reduced the red blood cell membrane fluidity index (fluorescence degree of polarization,average micro-viscosity,anisotropy) (P<0.05 or P<0.01),improved the activity of SOD,CAT and reduce the content of MDA (P<0.05 or P<0.01).[Conclusion] Oxymatrine may play a protective role on cerebral ischemia-reperfusion injury by improving hemorheology. |
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