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| Effects of epigallocatechin gallate against inflammatory injury in microglia based on TLT4-MyD88-TRAF6 signaling pathway |
| Hits 1517 Download times 1295 Received:November 20, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2019.03.22 |
| Key Words
epigallocatechin gallate;microglia;inflammation;signal pathway |
| Author Name | Affiliation | E-mail | | LIU Yan | Central of Health Management, Central Hospital of En-shi Autonomous Prefecture, Enshi 445000, China | | | ZHANG Yi | Central of Health Management, Central Hospital of En-shi Autonomous Prefecture, Enshi 445000, China | | | LEI Rui | Central of Health Management, Central Hospital of En-shi Autonomous Prefecture, Enshi 445000, China | | | HOU Hongying | Central of Health Management, Central Hospital of En-shi Autonomous Prefecture, Enshi 445000, China | 236528547@qq.com |
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| Abstract
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| [Objective] To observe the protective effects of epigallocatechin gallate (EGCG) on inflammatory injury in microglia,and investigate the effect of EGCG on the activation of TLT4-MyD88-TRAF6 signaling pathway.[Methods] LPS was used to induce the model of inflammatory injury in BV-2 cells in vitro. BV-2 cells were divided into five groups,including control group,LPS induced group,LPS+low dose (50 μmol/L) EGCG group,LPS+middle dose (100 μmol/L) EGCG group,and LPS+high dose (200 μmol/L) EGCG group. CCK8 assay was used to measure the relative survival rates of BV-2 cells. ELISA assay was used to detect the levels of interleukin 6 (IL-6),interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α). Western Blot was used to determine the expression levels of induced nitric oxide synthase (iNOS),cyclooxygenase (COX-2),Toll like receptor 4 (TLR4),Myeloid differentiation factor of 88 (MyD88) and tumor necrosis factor receptor related factor of 6 (TRAF6) proteins.[Results] Compared with control group,the relative survival rates of BV-2 cells were greatly icreased after treatment with different concentrations of EGCG (P<0.05). The levels of inflammatory factors (IL-6,IL-1β and TNF-α) were significantly decreased (P<0.05). The expression levels of iNOS,COX-2,TLT4,MyD88 and TRAF6 were also greatly decreased (P<0.05) with a dose-dependent manner.[Conclusion] EGCG can significantly inhibit the inflammatory response of BV-2 microglial cells induced by LPS via suppressing the activation of TLT4-MyD88-TRAF6 signaling pathway. |
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