|
| Study on the effects of hirudin on renal interstitial fibrosis and MCP-1,ICAM-1 in unilateral ureteral obstruction rats |
| Hits 1419 Download times 1068 Received:November 16, 2018 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2019.05.16 |
| Key Words
hirudin;renal interstitial fibrosis;MCP-1;ICAM-1 |
| Author Name | Affiliation | E-mail | | REN Tong | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | | | ZHU Pengyu | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | | | YANG Hongtao | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | elert324@126.com |
|
| Abstract
|
| [Objective] To observe the effect of hirudin on renal interstitial fibrosis in rats and to explore its mechanism.[Methods] The 120 healthy male Sprague-Dawley rats were randomly divided into sham operation group (SOR group) with 24 rats and model group with 96 rats. 96 rats were randomly divided into model group (UUO group) with 24 rats,high-hirudin group (UUO + HI group) with 24 rats,low-hirudin group (UUO + LI group) with 24 rats and benazepril group (UUO + LOT group) with 24 rats. Groups were randomly put to death on 3,7 and 14 days after operation. The obstructed lateral renal tissues were examined by RT-PCR and Western Blot. The molecular expressions of MCP-1 and ICAM-1 were detected.[Results] HE staining showed that,compared with the UUO group:the renal interstitial injury and fibrosis in each group were improved to varying degrees. RT-PCR showed that,compared to the UUO group:hirudin high dose group at each time point and hirudin low dose group on day 7,day 14 relative expression of ICAM-1 were significantly decreased (P<0.05);high hirudin dose group on day 7,day 14 of MCP-1 was significantly decreased (P<0.01);hirudin high dose group on day 3 and MCP-1 expression of hirudin low dose group at each time point was significantly decreased (P<0.05). Western Blot showed that compared with UUO group:ICAM 1-protein expression on day 7 hirudin high dose group was significantly reduced (P<0.01);hirudin high dose group on day 3,day 14 and hirudin low dose group day 14 at each time point ICAM-1 protein expression decreased significantly (P<0.05);MCP-1 protein expression on the 14th day hirudin high-dose group was significantly decreased (P<0.01);hirudin high dose group on day 3,MCP-1 expression on day 7 was significantly decreased (P<0.05).[Conclusions] Hirudin can improve renal interstitial injury and delay the progress of interstitial fibrosis in rats with UUO to varying degrees,which may be related to the intervention of hirudin in the expression of MCP-1,ICAM-1 mRNA and protein in renal interstitium of UUO rats. |
|
|
|
|
|
|
|
