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| Effects of resveratrol against inflammatory injury of human renal proximal tubular cells induced by LPS through the inhibition of TXNIP/NLRP3 signaling pathway |
| Hits 1471 Download times 1134 Received:December 29, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2019.05.20 |
| Key Words
resveratrol;human renal proximal tubular cells;inflammatory response;TXNIP/NLRP3 signaling pathway |
| Author Name | Affiliation | E-mail | | CUI Yonghe | Pharmacology Department, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang 441021, China | | | SHEN Xianmin | Pharmacology Department, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang 441021, China | jfc55555@163.con |
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| Abstract
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| [Objective] To observe the protective effects of resveratrol against inflammatory damage of HK-2 cells induced by LPS,and observe the effect of resveratrol on the activation of TXNIP/NLRP3 signaling pathway.[Methods] LPS (1 mg/L) was used to induce the model of inflammatory injury in HK-2 cells in vitro. HK-2 cells were divided into five groups,including control group,LPS-induced group,LPS+low dose (50 μmol/L) resveratrol group,LPS+middle dose (100 μmol/L) resveratrol group,and LPS+high dose (200 μmol/L) resveratrol group. CCK8 assay was used to measure the relative survival rates of HK-2 cells. ELISA assay was used to detect the levels of IL-6,IL-1β and TNF-α. Western Blot was used to determine the expression levels of iNOS,COX-2,TXNIP and NLRP3 proteins.[Results] Compared with LPS induced group,the relative survival rates of HK-2 cells were greatly increased after treatment with different concentrations of resveratrol (P<0.05). The levels of inflammatory factors (IL-6,IL-1β and TNF-α) were significantly decreased (P<0.05). The expression levels of iNOS,COX-2,TXNIP and NLRP3 were also greatly decreased (P<0.05) with a dose-dependent manner.[Conclusion] Resveratrol can significantly inhibit the inflammatory response of HK-2 cells induced by LPS via suppressing the activation of TXNIP/NLRP3 signaling pathway. |
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