|
| Astragaloside IV attenuates high glucose-induced H9c2 cardiomyocyte injury by inhibiting IKK/NF-κB inflammatory pathway |
| Hits 2174 Download times 1753 Received:January 02, 2019 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2019.06.20 |
| Key Words
astragaloside IV;high glucose;cardiomyocytes;inflammation;NF-κB |
| Author Name | Affiliation | | ZHANG Shuchun | Tangshan Nanhu Hospital, Tangshan 063000, China | | DAI Ziyang | North China University of Science and Technology, Tangshan 063210, China | | WANG Ya | North China University of Science and Technology, Tangshan 063210, China | | XIA Juan | Tangshan Nanhu Hospital, Tangshan 063000, China | | DU Mengfan | North China University of Science and Technology, Tangshan 063210, China | | YAO Wen | North China University of Science and Technology, Tangshan 063210, China |
|
| Abstract
|
| [Objective] To observe the mechanism of astragaloside IV in alleviating H9c2 cell injury induced by high glucose.[Methods]Injured model of H9c2 cardiomyocyte was induced by high glucose. Cell viability was determined by MTT assay. TNF-α and IL-6 levels in supernatant of cell culture medium were detected by ELISA. IKK-β,NF-κB,p-NF-κB and TNF-α were detected by Western blot.[Results] High glucose treatment on H9c2 cardiomyocytes significantly down-regulated cell viability at 12 h (P<0.05);pretreatment with astragaloside (20,40,80 μmol/L) for 10 min significantly maintained cell viability,reduced the content of TNF-α and IL-6 in supernatant of cell culture medium (P<0.05),and inhibited the protein expression of IKK-β and TNF-α (P<0.05),and the activation of p-NF-κB (P<0.05).[Conclusion] Astragaloside IV alleviates high glucose-induced H9c2 cardiomyocyte injury by inhibiting the excessive activation of IKK/NF-κB inflammatory pathway. |
|
|
|
|
|
|
|
