|
| Protective effect and mechanism study of Breviscapine on acute kidney injury induced by gentamicin |
| Hits 1931 Download times 964 Received:January 28, 2019 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2019.06.21 |
| Key Words
Breviscapine;kidney injury;gentamicin;renal function;inflammation |
| Author Name | Affiliation | | ZHAO Liya | Handan Central Hospital, Handan 056001, China | | HU Hongying | Handan Central Hospital, Handan 056001, China | | REN Libin | Handan Central Hospital, Handan 056001, China |
|
| Abstract
|
| [Objective] To investigate the protective effect of Breviscapine (Bre) on acute kidney injury (AKI) induced by gentamicin and its possible mechanism.[Methods] The 120 experimental Wistar rats were randomly divided into normal control group,model control group,Nephritis rehabilitation tablets group[800 mg/(kg·d),positive drug control group],Bre low-[75 mg/(kg·d)],medium-[150 mg/(kg·d)],high-[300 mg/(kg·d)] dose group,n=20;the AKI rat models were made by intraperitoneal injection of gentamicin. The drugs were continuously given for 14 days (once a day) and the rats in normal control group were given synchronous saline. Collecting and measuring 24-hour urine volume,and the activity of 24 h urine protein (Pro) and N-acetyl-β-glucosaminidase (NAG enzyme) were determined;the content of serum urea nitrogen (BUN),serum creatinine (Scr) were determined by biochemical analysis;the body weight,kidney weight were weighted and the kidney index was calculated;the expression of renal tissue damage-1 (KIM-1) was examined by immunohistochemical method (IHC). The pathological changes of renal tissue were observed by HE staining. The activities of oxidative stress index and inflammatory cytokines in renal tissue were measured.[Results] Compared with model control group,the 24 h urine output,24 h Pro of Bre medium-/high-dose groups and Nephritis rehabilitation tablets group were significantly decreased and the activity of NAG was significantly decreased(P<0.05 or P<0.01). The content of BUN,Scr was significantly decreased. The renal weight,renal index were significantly decreased;the expression of KIM-1 were down-regulated. Both of renal tissue structure lesions were significantly improved,the damage score were significantly reduced;the activity of SOD,CAT in renal tissue were significantly increased and the level of MDA were decreased;the content of MIP-2,MCP-1,TNF-α were significantly decreased;all of the difference above were significant (P<0.05 or P<0.01).[Conclusion] Bre has a protective effect on kidney injury induced by gentamicin in AKI rats,which may be related to alleviating renal tissue damage,anti-oxidation and inhibiting inflammation. |
|
|
|
|
|
|
|
