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| In vitro study on the antioxidant activities of Shenmai Injection |
| Hits 1560 Download times 1349 Received:December 13, 2018 |
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| DOI
10.11656/j.issn.1672-1519.2019.07.18 |
| Key Words
antioxidant;Shenmai Injection;DPPH free radical;Nitric oxide free radical;hydrogen peroxide;ferrous ion |
| Author Name | Affiliation | E-mail | | XIAO Wenhai | Dazu Chinese Medical Hospital, Chongqing 402360, China | | | YANG Zhengwei | Dazu Chinese Medical Hospital, Chongqing 402360, China | 10981668@qq.com | | LI Yuan | Central Laboratory of Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China | |
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| Abstract
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| [Objective] To systematic explore the antioxidant activities of Shenmai Injection. [Methods] The total antioxidant capability,DPPH· scavenging activity,NO· scavenging activity,H2O2 scavenging activity and Fe2+ chelating activity of the Shenmai Injection,which was diluted by different rates,were determined by Ferrric reducing antioxidant power assay (FRAR),DPPH· scavenging assay,NO· scavenging assay,H2O2 scavenging assay and Fe2+ chelating assay respectively. The results were compared with those of 0.1 mg/mL BHT,10 mmol/L Trolox and 10% citric acid. [Results] Shenmai Injection has certain antioxidant capacity in vitro. The total antioxidant capacity of Shenmai Injection was lower than that of BHT and Trolox (P<0.01),but higher than that of citric acid. The free radical scavenging activities of Shenmai Injection were positive concentration-dependence and were different for different free radical types. The order is NO·[(54.4±6.6)%] > Fe2+ ion[(42.6±3.2)%] > DPPH·[(36.2±2.8)%] > H2O2[(23.5±1.2)%]. [Conclusion] The in vitro experimental data of the antioxidant activities of Shenmai Injection will provide data support for its pharmacological mechanisms. |
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