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| Mechanisms research of anti-apoptosis by Danhong Injection in H9c2 myocardial cells |
| Hits 1359 Download times 1135 Received:March 15, 2019 |
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| DOI
10.11656/j.issn.1672-1519.2019.08.17 |
| Key Words
Danhong Injection;myocardial cell apoptosis;mitochondrial membrane potential;ROS;Ca2+ |
| Author Name | Affiliation | E-mail | | NIU Zichang | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | QIN Xiaoyan | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | HAN Xiaoling | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Jie | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WEI Qiu | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | CHEN Lu | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | 18502295944@163.com | | MAO Haoping | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | haoping_mao@126.com |
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| Abstract
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| [Objective] The aim of this article is to study the effects of Danhong injection on apoptosis induced by isoproterenol(ISO) in H9c2 myocardial cells from the perspective of mitochondrial damage.[Methods] H9c2 cells were cultured and there were control group,10 μmol/L ISO model group,10 μmol/L ISO group,20 μL/L Danhong injection group,200 μL/L Danhong injection group. Cell apoptosis was detected by flow cytometry. Mitochondrial membrane potential was detected by living cell fluorescence imaging. Production of reactive oxygen spices (ROS) was detected by laser focal microscope. Fluo-3,AM ester fluorescent dye were used to detect intracellular calcium concentration.[Results] Compared with control group (1.33±0.42)%,the apoptotic rate in ISO group increased (5.13±0.15)% (P<0.01);Compared with ISO group,the apoptotic rate in 20 and 200 μL/L Danhong injection groups decreased to(4.23±0.15)% and (2.37±0.38)% respectively. Compared with the control group,the degree of mitochondrial membrane potential depolarization increased significantly after ISO treatment,which was (151.76±16.01)% (P<0.01). 20 and 200 mL/L Danhong injection significantly inhibited the mitochondrial membrane depolarization induced by ISO,which were (121.07±11.53)% and (117.15±12.91)%. In addition,Danhong injection significantly inhibited the increase of ROS and Ca2+ concentration induced by ISO,and the differences were statistically significant.[Conclusion] Danhong injection can inhibit the depolarization of mitochondrial membrane potential induced by ISO and decrease the concentration of ROS and Ca2+ in cells,thus exerting its anti-apoptotic effect on H9c2 cells. |
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