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| Pharmacokinetic study of phenolic acids in rat plasma after oral administration of Qili Qiangxin Capsule |
| Hits 1294 Download times 1285 Received:March 29, 2019 |
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| DOI
10.11656/j.issn.1672-1519.2019.08.19 |
| Key Words
Qili Qiangxin Capsule;HPLC-MS/MS;phenolic acids;pharmacokinetics |
| Author Name | Affiliation | E-mail | | ZHANG Yu | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Fugeng | Tianjin Huanhu Hospital, Tianjin 300350, China | | | ZHU Mingdan | Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | | | ZHANG Shaoqiang | Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | | | DU Wuxun | Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China | cnduwux@163.com | | XIAO Xuefeng | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | kai1219@163.com |
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| Abstract
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| [Objective] A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed to study the pharmacokinetic characteristics of phenolic acids (salvianolic acid A,salvianolic acid B,danshensu,lithospermic acid,rosmarinic acid,protocatechuic aldehyde) in rat plasma after oral administration of Qili Qiangxin Capsule.[Methods] An Agilent ZOBRAX XDB-C18 column (4.6 mm×50 mm,3.5 μm) was used for the determination of analyte. And mobile phase was consisted of[0.1% formic water (A)] and[acetonitrile containing 0.1% formic acid (B)] for gradient elution,the flow rate was 0. 45 mL/min. The analytes were detected by multiple reaction monitoring (MRM) mode by negative ion mode in multiple reaction monitoring with electrospray ionization (ESI).[Results] The methodological results met the requirements. The pharmacokinetic parameter Tmax was 10~40 min,and t1/2 was 3.34~8.38 h. Cmax and AUC0-∞ were in the order of salvianolic acid B > danshensu > lithospermic acid > rosmarinic acid > protocatechuic aldehyde > salvianolic acid A.[Conclusion] The method was proved to be specific and repeatable,and to be suitable for determination of phenolic acids in rat plasma for pharmacokinetic study. |
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