|
| Association among oxidized low density lipoprotein and the expression of Notch1,Notch2 in CD4+ T cells in patients with acute coronary syndrome |
| Hits 1174 Download times 1051 Received:April 17, 2019 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2019.09.08 |
| Key Words
acute coronary syndrome;oxidized low density lipoprotein;Th1/Th2 cell;Notch1;Notch2 |
| Author Name | Affiliation | E-mail | | ZHU Jiaping | Department of Medicine, Xiangyang Central Hospital Affiliated Hospital of Hubei University of Arts and Science, Xiangyang 441021, China | | | ZHU Liru | Department of Medicine, Xiangyang Central Hospital Affiliated Hospital of Hubei University of Arts and Science, Xiangyang 441021, China | hubei8888xx@163.com |
|
| Abstract
|
| [Objective] To study the association between oxidized low density lipoprotein (ox-LDL) and the expression of Notch1,Notch2 in CD4+ T cells in patients with acute coronary syndrome.[Methods] The 26 patients with acute myocardial infarction (AMI),30 patients with unstable angina (UA),32 patients with stable angina (SA) and 30 healthy subjects (NC) were recruited. The plasma level of ox-LDL was measured by ELISA,and the ratio of Th1/Th2 and the expression of Notch1,Notch2 in CD4+ T cells in peripheral blood were detected by flow cytometry. Then CD4+ T cells were sorted by flow cytometry,T cells were cocultured with varying concentrations of ox-LDL (0,40,80,160 mg/L),the expression of Notch1 and Notch2 in CD4+ T cells were detected by flow cytometry in each group after 72 h.[Results] The expression levels of Notch1,Notch2 in CD4+ T cells were greatly decreased,the ratio of Th1/Th2 and the level of ox-LDL were significantly increased in AMI group and UA group compared with NC group (P<0.05). Pearson correlation analyses indicated that ox-LDL was significantly and negatively associated with the expression of Notch1 (r=-0.653,P<0.01) and Notch2 (r=-0.468,P<0.01) in patients with acute coronary syndrome,and ox-LDL was significantly and positively associated with the ratio of Th1/Th2 (r=0.582,P<0.01).[Conclusions] ox-LDL can obviously inhibit the expression of Notch1 and Notch2 in human peripheral blood CD4+ T cells,which may be the reason that ox-LDL inhibit the differentiation of Th2 and promote the differentiation of Th1,and participate in the occurrence and development of atherosclerosis. |
|
|
|
|
|
|
|
