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| Study on Shenshuning's intervention of HBV-induced transdifferentiation and migration of HK-2 Cells |
| Hits 1201 Download times 904 Received:April 19, 2019 |
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| DOI
10.11656/j.issn.1672-1519.2019.09.18 |
| Key Words
Shenshuning;hepatitis B virus-associated nephritis;transdifferentiation;Transwell |
| Author Name | Affiliation | E-mail | | YANG Xikai | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | | | XU Bing | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Yaoguang | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | wangyaoguang1012@126.com |
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| Abstract
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| [Objective] To investigate the effect of Shenshuning on the transdifferentiation of HK-2 cells induced by HBV plasmid.[Method] Mouse drug-containing serum was prepared. HK-2 cells were cultured and transfected with HBV plasmid. The cells were divided into:blank serum control group,empty plasmid group,HBV group,Shenshuining low concentration group,Shenshuinging middle concentration group,and Shenshuing high concentration group. The expression of HBsAg,HBeAg,α-SMA,E-cadherin and cell migration ability in HK-2 cells were detected by enzyme-linked immunosorbent assay (ELISA),immunocytochemistry and Transwell cell migration assay.[Results] ELISA detection:HBsAg and HBeAg expression were up-regulated in HBV group compared with empty plasmid group and blank serum group (P<0.01). Immunocytochemical detection:compared with the HBV group,the expression of α-SMA protein in the low-concentration group of Shenshuening,the concentration of Shenshuening and the high concentration of Shenshuing was significantly down-regulated,and the difference was significant (P<0.01). Compared with the HBV group,the low-concentration group of Shenshuing,the concentration of Shenshuing and the high concentration of Shenshuing had an up-regulation of E-cadherin protein expression,but the difference was not statistically significant (P>0.05). Compared with the HBV group,the migration ability of the kidney-sparing low-concentration group,the Shenshuing-Ning-concentration group,and the Shenshu-ning high-concentration group was significantly weakened,and the difference was significant (P<0.01).[Conclusion] HBV transfection of HK-2 cells will cause cell transdifferentiation and enhance cell migration ability. The drug-containing serum of Shen Shu Ning can alleviate the transdifferentiation of epithelial cells caused by HBV and reduce its cell migration ability. |
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