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| Research of the effect of Xifeng Capsule on the function of voltage-gated sodium channel in epileptic rats induced by pilocarpine |
| Hits 1464 Download times 967 Received:February 23, 2019 |
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| DOI
10.11656/j.issn.1672-1519.2019.10.17 |
| Key Words
Voltage-gated sodium channel;Xifeng Capsule;epilepsy;whole cell patch clamp |
| Author Name | Affiliation | E-mail | | LU Yanli | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | FANG Yanyan | Chinese Medicine Hospital of Linyi Shandong Province, Linyi 276002, China | | | LI Xinmin | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | lixinmin1998@sohu.com | | SUN Dan | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | MA Liting | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HAN Yaowei | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | |
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| Abstract
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| [Objective] To study he effect of Xifeng Capsule on the function of voltage-gated sodium channel in hippocampus of epileptic rats induced by lithium chloride and pilocarpine.[Methods] The epileptic rat model was induced by lithium chloride and pilocarpine. The experimental rats were randomly divided into 5 groups:blank group,model dose group,low dose group of low dose group of Xifeng Capsules (low dose group),medium dose group of Xifeng Capsules (medium dose group),and high dose group of Xifeng Capsules (high dose group). VGSC function of hippocampal nerve cell was detected through the whole cell patch clamp techniques.[Results] 1)lite-pirocarpine rat model was duplicated successfully. Compared with model dose group,the number of episode times of high dose group,extinguish the episodes and low dose group were less than model dose group (P<0.01). With the increase of the dose,the number of episode times reduced gradually,and there was statistically significance in the number of episode times between high dose group and low dose group (P<0.05). 2)Total cell patch clamp to record sodium current results:compared with the blank group,the sodium current density of each group significantly was increased,the threshold value of activation curve was decreased,the threshold value of inactivation curve was increased,and the recovery time after inactivation was shortened,and there were statistically significance in all these results (P<0.01). Compared with the model group,the sodium current density of all Xifeng Capsule groups were decreased,activation curve threshold were increased,inactivation curve threshold were decreased,and recovery time were prolonged,and there were statistically significance in all these results (P<0.01). Compared with the medium dose group,the current of the high dose group tended to decrease,but there was no statistical significance in two groups (P>0.05),while compared with the low dose group,the current of the medium dose group and the high dose group showed significant difference (P<0.01). As to the activation curve and inactivation in all dose groups,with the increase of the dose,the value of activation and inactivation tended to decrease,but there was no statistical significance between the treatment groups (P>0.05). The recovery curve of inactivation showed an overall extension tendency with the increase of dose recovery time,but there was no statistical significance between the treatment groups (P>0.05).[Conclusion] Xifeng Capsule may reduce the abnormal activation of sodium channel by reducing sodium channel current,increasing the inactivation of sodium channel,inhibiting the recovery after inactivation,and prolonging the recovery time,thus reducing recurrent spontaneous seizures,which may be one of its mechanisms. |
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