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| Determination of the trans-anethole content in fructus foeniculi and its variation rule |
| Hits 1278 Download times 1076 Received:May 29, 2019 |
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| DOI
10.11656/j.issn.1672-1519.2019.10.20 |
| Key Words
Fructus Foeniculi;ultra performance liquid chromatography;trans-anethole |
| Author Name | Affiliation | E-mail | | WANG Zuoyuan | Institute of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Min | Institute of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | YANG Jing | Institute of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | yangj0622@tjutcm.edu.cn | | CHAI Xin | Institute of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Yuefei | Institute of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | |
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| Abstract
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| [Objective] To establish a method for determining the trans-anethole content in Fructus Foeniculi by ultra-performance liquid chromatography (UPLC). The variation rule of trans-anethole content in Fructus Foeniculi (raw) and its powder over time was systematically studied.[Methods] The analysis was performed on ACQUITY UPLC®BEH C18 Column (2.1 mm×50 mm,1.7 μm) with the mobile phase of methanol-0.1% formic acid (55:45) at 0.3 mL/min. The detection wavelength was 254 nm. The column temperature was fixed at 40℃,the temperature of sample injector was 5℃ and the injection volume was 2 μL. Samples of Fructus Foeniculi (raw) and its powder placed at room temperature were collected at the specified times,and the trans-anethole content of which was analyzed.[Results] The methodological study for determination of trans-anethole in Fructus Foeniculi satisfied the requirements of Validation Guidelines for Analytical Method of Pharmaceutical Quality Standard from ChP. The trans-anethole content in Fructus Foeniculi (raw) and its salt product was quite different,almost half of which cannot meet the standard released by ChP. The trans-anethole content in the salt product was slightly changed during the processing. Under the condition of room temperature,the trans-anethole content in Fructus Foeniculi (raw) was basically stable within the specified storage time. However,the trans-anethole content decreased rapidly within the specified storage time after the Medicinal herbs were pulverized.[Conclusion] The method has the advantages of simple pretreatment,good reproducibility,fast analysis,accurate and reliable result,which can be used for studying the trans-anethole content in Fructus Foeniculi and its variation rule. |
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