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| Optimization of preparation process for celery seeds oil-hydroxypropyl-β-cyclodextrin inclusion complex by central composite design-response surface methodology |
| Hits 2283 Download times 1267 Received:June 29, 2019 |
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| DOI
10.11656/j.issn.1672-1519.2019.10.21 |
| Key Words
celery seed oil;butylphthalide;central composite design-response surface methodology;inclusion process |
| Author Name | Affiliation | E-mail | | LIU Daoqun | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | GUO Daliang | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHAO Yu | Tianjin Darentang Jingwanhong Pharmaceutical Co., Ltd., Tianjin 300112, China | | | LIU Yan | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | SONG Xiuning | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LIU Yuxuan | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | LsrLyx2008@126.com |
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| Abstract
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| [Objective] To optimize inclusion process for celery seeds oil with hydroxypropyl-β-cyclodextrin (HP-β-CD). Inclusion compound was also characterized and evaluated.[Methods] Inclusion complex was prepared by freeze-drying. Inclusion ratio,yield,contents of butylphthalide were the evaluated,and comprehensive score value was calculated. Inclusion temperature,inclusion time and feed ratio were investigated by single factor and three-factor five-level central composite design. The results were fitted by second or three-order polynomial multi-linear formulation. The predicted inclusion process was certificated to be optimal. Inclusion complex was determined by HPLC,UV and DSC.[Results] The optimal inclusion temperature was 44.5℃,the inclusion time was 167 min,and feed ratio of celery seed oil and HP-β-CD was 2:25. Inclusion rate of volatile oil was 78.95%,and the content of butylphthalide in complex was 2.982 mg/g. Characterization results showed that celery seed oil was successfully encapsulated by HP-β-CD.[Conclusion] The central composite design-response surface methodology was suitable to optimize the inclusion process. The mathematical model showed good predictability. |
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