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| Mechanism of treatment on myelodysplastic syndrome with Q-PCR detection of TRAF3 gene pathway under the function of Qinghuang Powder |
| Hits 1494 Download times 1175 Received:July 08, 2019 |
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| DOI
10.11656/j.issn.1672-1519.2019.11.23 |
| Key Words
myelodysplastic syndrome;TRAF;Q-PCR |
| Author Name | Affiliation | | TANG Xudong | Department of Hematology, Xiyuan Hospital China Academy of Chinese Medical Sciences, Beijing 100091, China | | ZHANG Lu | School of Management, Beijing University of Chinese Medicine, Beijing 100029, China | | YANG Xiupeng | Department of Hematology, Xiyuan Hospital China Academy of Chinese Medical Sciences, Beijing 100091, China | | TANG Yufeng | Laboratory, Xiyuan Hospital China Academy of Chinese Medical Sciences, Beijing 100091, China | | WANG Dexiu | Graduate School, Beijing University of Chinese Medicine, Beijing 100029, China |
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| Abstract
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| [Objective] To reveal the mechanism of Qinghuang Powder in the treatment of myelodysplastic syndrome (MDS) by using Quantitative PCR to detect the change of TRAF3 pathway gene in human myelodysplastic syndrome cells (MUTZ-1) after treatment with realgar and indirubin (indigo naturalis extract).[Methods] MUTZ-1 was cultured according to group A as blank control group,group B was realgar group (concentration 1 μmol/L),group C was realgar (concentration 1 μmol/L)+high dose indirubin (concentration 50 μmol/L),group D was realgar (concentration 1 μmol/L) + low dose indirubin (concentration 0.05 μmol/L),and the IFN-γ,TRAF3,IL10,IRF3,RIP and GAPDH genes were detected by Q-PCR.[Results] The expression of TRAF3 gene in group B was low,in group C and D was high,and group B,C and D were lower than group A (P<0.05). The expression of RIP gene in B group was high,in group C and D was low,and group B,C and D were higher than group A (P<0.05). The expression of IRF3 gene in group D was high,in group B and C was low,and group B,C and D were lower than group A (P<0.05). IL-10 gene was highly expressed in group C,followed by group B and low in group D,and group B,C and D were lower than group A (P<0.05). IFN-γ gene was highly expressed in group D,followed by group C and low in group B,and group D were higher than group A (P<0.05).[Conclusion] Qinghuang Powder (combination of Ming realgar and indirubin) can increase the expression of RIP gene when the concentration of indigo naturalis is low,and the expression of TRAF3,IRF3,IL-10 and IFN-γ genes is reduced. When the concentration of indigo naturalis is high,it can cause the increase of RIP and IFN-γ genes expression,and the expression of IRF3,TRAF3 and IL-10 genes can be reduced,which can promote the production of IFN-γ and enhance the killing ability of NK cells against tumor cells. With the increase of the concentration of indigo naturalis,the IFN-γ gene continues to be up-regulated,IL-10 continues to be down-regulated,and the killing ability of NK cells to tumor cells continued to be enhanced. Therefore,in the future,it is possible to affect TRAF3 and its signaling pathway of the MUTZ-1 human cell line by adjusting the concentration of the indigo naturalis solution,so as to achieve a good therapeutic purpose. |
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