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| Study on the apoptosis of gastric cancer cells induced by sulforaphane mediated Wnt/β-catenin signaling pathway |
| Hits 1546 Download times 904 Received:July 25, 2019 |
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| DOI
10.11656/j.issn.1672-1519.2019.11.25 |
| Key Words
sulforaphane;gastric cancer cell;proliferation;apoptosis;Wnt/β-cateni signaling pathway |
| Author Name | Affiliation | E-mail | | LI Li | Enshi Tujia and Miao Autonomous Prefecture Central Hospital Operating Room, Enshi 445000, China | | | GU Wenyan | Enshi Tujia and Miao Autonomous Prefecture Central Hospital Operating Room, Enshi 445000, China | | | JIANG Hong | Enshi Tujia and Miao Autonomous Prefecture Central Hospital Operating Room, Enshi 445000, China | 574423227@qq.com |
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| Abstract
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| [Objective] To investigate the effect of sulforaphane on the proliferation of gastric cancer SGC-7901 cells and its effect on the activation of Wnt/β-catenin signaling pathway.[Methods] The effects of 10,20,40 μmol/L sulforaphane on the proliferation of SGC-7901 cells were detected by CCK8 assay. The effect of sulforaphane on the apoptosis of SGC-7901 cells was analyzed by flow cytometry. The luciferase reporter gene assay was used to detect the effect of sulforaphane on the transcriptional activity of lymphatic enhancement factor/lymphatic adhesion factor (TCF4/LEF) in Wnt/β-catenin pathway. Western Blot method was used to detect the effect of sulforaphane on the β-catenin,glycogen synthase kinase 3β,c-Myc and Caspase 3 protein expression levels in SGC-7901 cells.[Results] Compared with the control group,the inhibition rate of SGC-7901 cells in the sulforaphane group was significantly increased (P<0.05),showing a time-and dose-dependent manner;the apoptosis rate of SGC-7901 cells in the sulforaphane group was significantly higher than that in the control group (P<0.05),and sulforaphane could also significantly inhibit the luciferase activity of TCF4/LEF reporter plasmid (P<0.05). The expression of β-catenin and c-Myc protein in SGC-7901 cells of sulforaphane group was concentration-dependent increase,while the expression of GSK-3β and Caspase3 protein increased in a concentration-dependent manner (P<0.05).[Conclusion] The sulforaphane can significantly inhibit the proliferation and induce apoptosis of SGC-7901 cells,and its mechanism may be related to the inhibition of Wnt/β-catenin signaling pathway activation. |
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