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| Effect of Qidan Lixin Pill on myocardial fibrosis in rats with chronic heart failure after myocardial infarction |
| Hits 1356 Download times 885 Received:July 18, 2019 |
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| DOI
10.11656/j.issn.1672-1519.2019.12.18 |
| Key Words
TGF-β1/Smads signaling pathway;Yiqi Huoxue Lishui;Qidan Lixin Pill;myocardial infarction;chronic heart failure;myocardial fibrosis |
| Author Name | Affiliation | E-mail | | SHI Binhao | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | HUANG Yuting | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | XU Zongpei | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | FAN Guanwei | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | fgw1005@hotmail.com |
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| Abstract
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| [Objective] To study the effect of Qidan Lixin Pills (QD) on myocardial fibrosis in rats with chronic heart failure (CHF) after myocardial infarction (MI).[Methods] CHF was induced by the left anterior descending artery ligation (LAD) in adult male Sprague-Dawley rats. After 4 weeks of intervention with QD (1.1,2.2,4.4 g/kg·d),cardiac function indexes were measured by echocardiography and pressure volume system. The masson staining was used to compare the changes of pathological fibrosis in each group. The mRNA levels of collagen I and collagen III in each group were measured by RT-PCR. Western blot was used to detect the expression of TGF-β1,Smad3 and Smad7 proteins.[Results] At the end of the experiment,the indexes of cardiac function (LVEF,LVFS,+dp/dt max,-dp/dt max) were significantly reduced in model group (P<0.05). The ventricular remodeling was obviously changed. The QD can improve the cardiac function and delay the process of ventricular remodeling of chronic heart failure rats. Among them,the improvement of cardiac function was more obvious in the middle-dose group and high-dose group,and ventricular remodeling was relieved (P<0.05). QD also inhibited the expression of Collagen I and III (P<0.05),reduced the formation of myocardial fibroblasts,down-regulated the expression of myocardial fibrosis related proteins TGF-β1 and Smad3 (P<0.05),and increased the level of anti-fibrosis protein Smad7 (P<0.05).[Conclusion] Yiqi Huoxue Lishui Prescription (Qidan Lixin Pills) can improve cardiac function in chronic heart failure rats after myocardial infarction,delay ventricular remodeling and myocardial fibrosis. Its mechanism may be related to regulating TGF-β1/Smads signaling pathway. |
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