|
| Effect of Guanmaitong Tablet on prevention and treatment of vascular calcification in rats with chronic kidney disease |
| Hits 1422 Download times 925 Received:July 24, 2019 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2019.12.19 |
| Key Words
Guanmaitong Tablet;bushen huoxue;chronic kidney disease;vascular calcification;rat |
| Author Name | Affiliation | E-mail | | WANG Hong | Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, China | | | SHEN Yuyan | Tianjin Tongrentang Group Co., Ltd., Tianjin 300385, China | | | YUAN Ling | Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, China | | | LIU Linna | Hospital of Traditional Chinease Medicine of Zhongshan, Zhongshan 528400, China | | | LI Xu | Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, China | | | CUI Xiaoxue | Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, China | | | ZHANG Fachang | Tianjin Tongrentang Group Co., Ltd., Tianjin 300385, China | | | WANG Lei | Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, China | zws9905@sina.com |
|
| Abstract
|
| [Objective] To observe the effects of Guanmaitong tablets on the prevention and treatment of vascular calcification in rats with chronic kidney disease (CKD).[Methods] Vascular calcification model with CKD in rats was prepared by gavage with adenine and high phosphorus water. Different dosages of Guanmaitong tablets were given by gavage for 10 weeks. The levels of serum urea,creatinine,calcium,phosphorus and the content of calcium in the abdominal aorta were detected. Renal and vascular tissue changes were observed by HE staining. Von kossa stainings were used to observe the degrees of vascular calcification.[Results] The levels of serum urea,creatinine and phosphorus in the model rats were significantly reduced,the level of calcium was increased and the content of calcium in abdominal aorta was obviously decreased in the groups of Guanmaitong tablets 0.46,0.23 g/kg. HE staining showed that compared with the normal group,disordered glomerular structure,tubular ectasia,brown crystal deposits were seen locally. The interstitium of the kidney was permeated with lymphatic and mononuclear infiltration,and a large number of fibroblasts proliferated,with extensive fibrosis and significantly reduced renal vessels. Compared with the model group,the degree of renal tubule dilation decreased,the number of inflammatory cells decreased,and adenine crystals decreased,with the effect in the group of 0.46 g/kg was better. HE and Von kossa stainings showed that compared with the model group,the area of calcification lesion in the thoracic aorta of rats in the groups of Guanmaitong tablets 0.46,0.23 g/kg was significantly reduced,and the effect in the group of 0.46 g/kg was better.[Conclusion] Guanmaitong tablets had significant prevention and treatment effects on vascular calcification in rats with CKD,which could improve renal function,correct the disorder of calcium and phosphorus metabolism,reduce the content of calcium and the area of calcification in arteries. |
|
|
|
|
|
|
|
