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| Study on the influence of Tangerine and Amomum on Rehmannia glutinosa-processed on the content of Verbascoside |
| Hits 1395 Download times 915 Received:July 25, 2019 |
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| DOI
10.11656/j.issn.1672-1519.2019.12.22 |
| Key Words
Tangerine Peel;Amomum;rehmannia glutinosa;Verbascoside glycoside;UPLC-MS |
| Author Name | Affiliation | | WANG Zhonghua | Department of Pharmacy, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | DOU Zhiying | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | WANG Yang | Department of Pharmacy, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | CHEN Tao | Department of Pharmacy, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | WANG Yuanzhi | School of Economics, Nankai University, Tianjin 300071, China |
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| Abstract
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| [Objective] According to the medicinal characteristics of Rehmannia glutinosa,we studied the effects of Tangerine Peel and Amomum on the content of Verbascoside in the process of processing Rehmannia glutinosa. And established UPLC-MS method for detecting the content of Verbascoside in Radix Rehmanniae. According to its content change,the scientificity and rationality of the processing method was verified. It provided reference for enriching the products of Rehmannia glutinosa and establishing its quality control standards.[Methods] We refered to the processing method of Rehmannia glutinosa in the "Chinese Medicine Processing Regulations" of the nation and provinces and adopted the methods with clinical and practical characteristics as follows:the Tangerine Peel-processed Rehmannia glutinosa,and the Amomum-processed Rehmannia glutinosa (wine steaming method and mixing method). Based on 2015 edition of the Chinese Pharmacopoeia,the quality standard of Rehmannia glutinosa was used as a reference index. UPLC-MS was used to detect the content of Verbascoside in the process of processing. Combined with trait scores,the comprehensive evaluation and statistical analysis were operated.[Results] The linear range of detection was (1-1 000 ng/mL,r=1),and its precision,repeatability,stability and accuracy were stable (RSD<3%). The content of Verbascoside in the Rehmannia glutinosa after the experiment was more than the pharmacopoeia standard. When the trait reached the pharmacopoeia or traditional standard in the process of preparation,the content of Verbascoside in the experiment could reach at a high level. The content of the Tangerine Peel processed Rehmannia glutinosa was kept the highest level,and the content change was the most obvious during the processing.[Conclusion] The Tangerine Peel-processed Rehmannia glutinosa can obviously stabilize and control the downward trend of Verbascoside in the processing. Through the corresponding processing methods,it is of great significance to preserve the index components of Radix Rehmanniae and expand the clinical use range of Rehmannia glutinosa effectively. At the same time,UPLC-MS method can be used for the detection of Verbascoside content in the processing of Radix Rehmanniae due to its simple operation,high speed and high accuracy. |
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