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| Experimental study of Bushen Huatan Recipe on Leptin and β2-AR expression in castration osteoprosis rats |
| Hits 1271 Download times 849 Received:October 27, 2019 |
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| DOI
10.11656/j.issn.1672-1519.2020.01.19 |
| Key Words
Bushen Huatan Recipe;Leptin;β2 adrenergic receptor;postmenopausal osteoporosis |
| Author Name | Affiliation | E-mail | | LI Zhangqing | Hubei University of Chinese Medicine, Wuhan 430065, China | | | XIANG Nan | Hubei University of Chinese Medicine, Wuhan 430065, China | xiangnan61@sina.com | | ZHOU Guangwen | Hubei University of Chinese Medicine, Wuhan 430065, China | | | ZHANG Lin | Wuhan Hospital of Traditional Chinese and Western Medicine, Wuhan No.1 Hospital, Wuhan 430022, China | | | ZHANG Yan | Hubei University of Chinese Medicine, Wuhan 430065, China | | | TAN Zhangkui | Hubei University of Chinese Medicine, Wuhan 430065, China | | | HUANG Shiyi | Hubei University of Chinese Medicine, Wuhan 430065, China | |
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| Abstract
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| [Objective] To investigate the mechanism of Bushen Huatan Recipe on the expression of Leptin and β2-AR to prevent and treat postmenopausal osteoporosis.[Methods] The 75 rats were randomly divided into sham operation group,model group,Bushen Huatan group,livial group and atorvastatin group. The bilateral ovariectomy was used to establish the castration osteoporosis model. The sham operation group only the same quality adipose tissue around the ovary was removed. The bone density(BMD) of the right femur was measured by Micro-CT. The serum leptin and estradiol(E2) were detected by ELISA. Automatic biochemical analyzer for detection of triglyceride (TG),total cholesterol (TC),high density lipoprotein cholesterol (HDL-C),low density lipoprotein cholesterol (LDL-C). PCR detected the relative expression of Leptin mRNA,β2 adrenergic receptor(β2-AR) mRNA in bone tissue.[Results] The levels of BMD,serum Leptin and E2 in the model group were significantly lower than those in the sham operation group. After treatment,the levels of BMD,serum Leptin,E2,HDL-C and Leptin mRNA in the bone marrow of the Bushen Huatan group were significantly increased compared with those of the model group. Serum TC,TG,LDL-L and bone tissue β2-AR mRNA expression levels were significantly decreased,and there was no significant difference between the Livial group and the atorvastatin group.[Conclusion] Bushen Huatan Recipe can effectively improve the bone mineral density of postmenopausal osteoporosis rats,supplement estrogen levels,mediate the activity of sympathetic nerve by regulating the level of Leptin,prevent postmenopausal osteoporosis and improve lipid metabolism. |
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