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| Rapid determination of acetic acid,propionic acid and butyric acid in Shuxuetong Injection by gas chromatography |
| Hits 1090 Download times 834 Received:October 29, 2019 |
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| DOI
10.11656/j.issn.1672-1519.2020.01.21 |
| Key Words
Shuxuetong Injection;acetic acid;propionic acid;butyric acid;gas chromatography |
| Author Name | Affiliation | E-mail | | LI Lingyun | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | NI Kailing | Mudanjiang Youbo Pharmaceutical Co., LTD., Mudanjiang 150711, China | | | LI Min | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | HAN Lifeng | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | YANG Jing | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | yangj0622@tjutcm.edu.cn | | CHAI Xin | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Yuefei | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | |
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| Abstract
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| [Objective] A rapid method was established for determination of short-chain fatty acids(acetic acid,propionic acid and butyric acid) in Shuxuetong Injection by gas chromatography.[Methods] The contents of acetic acid,propionic acid and butyric acid in Shuxuetong Injection were determined by gas chromatography (GC) with 2-ethylbutyric acid as internal standard. Using DB-FFAP elastic quartz capillary column(30 m×0.25 mm×0.25 μm),the component analysis was realized within 18 min after programmed temperature rise. The high purity nitrogen (N2) was employed as carrier gas(1.0 mL/min) and make-up gas (25 mL/min),respectively. The temperature of inlet and FID detector was fixed at 240℃.[Results] Through the analysis of leeches and earthworm,the raw material of Shuxuetong Injection,it was found that both leeches and earthworm contained acetic acid,butyric acid and propionic acid. Through analysis of 36 batches of Shuxuetong Injection,it was found that the content of acetic acid,propionic acid and butyric acid was 382.2~544.6 μg per ampulla bottle,90.92~217.5 μg per ampulla bottle,39.74~64.27 μg per ampulla bottle,respectively. There is visible different on the contents of acetic acid,propionic acid and butyric acid in different batches of Shuxuetong injection,and the total content of these acids are relatively stable.[Conclusion] The method established in this study for the determination of acetic acid,propionic acid and butyric acid in Shuxuetong Injection is simple,rapid and sensitive. |
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