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| Protective effect and mechanism of morusin on D-galactose induced alzheimer disease in mice |
| Hits 1402 Download times 776 Received:October 27, 2019 |
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| DOI
10.11656/j.issn.1672-1519.2020.01.22 |
| Key Words
morusin;D-galactose;alzheimer disease;inflammation;apoptosis |
| Author Name | Affiliation | E-mail | | ZHONG Fangfang | First People's Hospital of Shangqiu, Shangqiu 476100, China | | | YAN Mingguang | First People's Hospital of Shangqiu, Shangqiu 476100, China | | | YANG Fang | First People's Hospital of Shangqiu, Shangqiu 476100, China | | | ZHU Ge | First People's Hospital of Shangqiu, Shangqiu 476100, China | | | YIN Weibing | First People's Hospital of Shangqiu, Shangqiu 476100, China | 475410026@qq.com |
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| Abstract
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| [Objective] To explore the protective effect of morusin on D-galactose induced alzheimer disease in mice and its possible mechanisms.[Methods] AD model was established by subcutaneous injection of D-galactose 100 mg/(kg·d) for 8 weeks. The dose of low-dose morusin group,middle-dose morusin group,high-dose morusin group and piracetam group were 10,20,40 mg/(kg·d),and 0.75 g/(kg·d). After 8 weeks of administration,the learning and memory ability of mice were tested by Morris water maze. The pathological changes of hippocampal dentate gyrus were observed by HE staining. The content of TNF-α and IL-1β in serum was detected. The activity of ChAT,the content of ACH and the expression of p53 protein in brain tissue were detected.[Results] Compared with the control group,the escape latency in the model group were significantly increased. The numbers of passing through the platform were obviously reduced (P<0.01). Neurons in the brain's dentate gyrus were severely damaged. The levels of TNF-α and IL-1β in serum were increased. The activity of ChAT and the content ACH in brain tissue were decreased(P<0.01). The expression of p53 protein was significantly increased (P<0.01). Compared with the model group,the escape latency in the morusin group were significantly decreased (P<0.05 or P<0.01). The numbers of passing through the platform were obviously increased (P<0.05 or P<0.01). Neurons in the brain's dentate gyrus were obviously improved. The levels of TNF-α and IL-1β in serum were decreased (P<0.05 or P<0.01). The activity of ChAT and the content ACH in brain tissue were increased(P<0.05 or P<0.01). The expression of p53 protein was significantly decreased (P<0.05 or P<0.01) and appeared dose-dependent. The above index were improved in piracetam group.[Conclusions] Morusin has protecting effects on D-galactose induced alzheimer disease in mice. The mechanism may be related to reducing inflammation,inhibiting apoptosis,increasing the content of ACH and the activity of ChAT in synapses. |
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