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| Pathogenesis research of coronary heart disease with phlegm-stasis syndrome rat model based on PI3K/Akt pathway |
| Hits 1166 Download times 762 Received:October 30, 2019 |
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| DOI
10.11656/j.issn.1672-1519.2020.02.21 |
| Key Words
coronary heart disease;phlegm and blood-stasis syndrome;PI3K/Akt;apoptosis;animal model |
| Author Name | Affiliation | E-mail | | GAO Shan | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Pengwei | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | FENG Man | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Shuo | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Lin | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | PAN Ye | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LIU Yijia | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | YU Chunquan | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | ycq-4@163.com |
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| Abstract
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| [Objective] To investigate the pathogenesis of the rat model of coronary heart disease with phlegm and blood-stasis syndrome based on PI3K/Akt pathway.[Methods] Wistar male rats were randomly divided into blank group,sham operation group,turbidity group,blood stasis group and phlegm and blood-stasis group. On the 54th day,the left anterior descending coronary artery of the rats in blood-stasis group and the phlegm and blood-stasis group were ligated,and the rats in the sham operation group only threaded without ligation. The rats in the blank group,the blood-stasis group and the sham operation group were fed with normal feed for 8 weeks,while the phlegm group and the phlegm and blood-stasis group were fed with high fat diet for 8 weeks. The apoptosis of cardiomyocytes was observed by Tunel staining. The expressions of Akt,p-Akt,Bax,Bcl-2 and Caspase-3 were detected by Western blot.[Results] The results of Tunel staining showed that the apoptosis of phlegm group,blood-stasis group and phlegm and blood-stasis group increased significantly. Western blot results showed that the expression levels of Akt,p-Akt and Bcl-2 protein in blood-stasis group and phlegm and blood-stasis group were significantly lower than those in the blank group (P<0.05 or P<0.01). The expression levels of Bax and Caspase-3 were significantly increased (P<0.05 or P<0.01). The expression of p-Akt protein in the phlegm group was significantly increased (P<0.05),and there was no significant difference in the expression of Akt,Bcl-2,Bax and Caspase-3.[Conclusion] It is suggested that PI3K/Akt pathway maybe one of the pathogenesis of coronary heart disease with phlegm and blood-stasis syndrome by down-regulating Akt,p-Akt and Bcl-2 proteins in cardiomyocytes,up-regulating Bax and Caspase-3 proteins. |
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