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| Biocompatibility evaluation of a puerarin-interact,injectable and Ca2+ responsive hydrogel |
| Hits 1202 Download times 781 Received:October 15, 2019 |
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| DOI
10.11656/j.issn.1672-1519.2020.02.22 |
| Key Words
puerarin;calcium alginate;ion responsive hydrogel;biocompatibility |
| Author Name | Affiliation | E-mail | | WANG Guifang | Chinese Medicine Research Institute, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | YUE Zhanlong | Chinese Medicine Research Institute, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | CUI Yuanlu | Chinese Medicine Research Institute, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | cuiyl@tju.edu.cn |
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| Abstract
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| [Objective] To evaluate the biocompatibility of calcium ion sensitive injectable hydrogel with puerarin for arthritis treatment in vivo.[Methods] The degradation of puerarin-calcium alginate hydrogel was investigated in rats,which was implanted with different formulations of puerarin-calcium alginate hydrogel. The protein expression of inflammatory cytokines such as IL-1β,IL-6,and TNF-α were determined by ELISA,and the mRNA expressions of IL-1β,IL-6 and TNF-α were analyzed by real time RT-PCR in the tissue around the hydrogel. HE staining was used to observe the pathological changes of tissues around the hydrogel at different times.[Results] There was no significant decrease of the hydrogel volume in different time,which indicated that the rate of the hydrogel degradation was very slow in vivo. Compared with the sham-operated group,the protein levels of IL-1 β,IL-6 and TNF-α were significantly decreased in puerarin-calcium alginate gel group,but the mRNA expression of IL-1β,IL-6 and TNF-α was not significantly changed. HE staining results showed that puerarin-calcium alginate hydrogel only produced short-term inflammatory response by physical damage in the subcutaneous connective tissue layer,but no inflammatory response after long-term implantation,which indicated that the hydrogel had good biocompatibility.[Conclusion] In this study,injectable puerarin-calcium alginate hydrogel with suitable strength was constructed,which has the characteristics of slow degradation and good biocompatibility in vivo. It is expected to be excellent drug and cell carriers for the treatment of osteoarthritis. |
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