|
| Effect of Huoxue Wentong Formula on oxidative stress and apoptosis of cardiomyocyte induced by hypoxia |
| Hits 1336 Download times 795 Received:October 23, 2019 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2020.03.20 |
| Key Words
Huoxue Wentong Formula;hypoxia;cardiomyocyte;reactive oxygen species;apoptosis |
| Author Name | Affiliation | E-mail | | LIU Tiantian | Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China | | | WANG Qingqing | Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China | | | YAO Kuiwu | Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China | yaokuiwu@126.com | | LIU Youming | Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China | | | DUAN Jinlong | Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China | |
|
| Abstract
|
| [Objective] To observe the protective effect of Huoxue Wentong Formula on hypoxia-induced injury of H9c2 myocardial cells.[Methods] H9c2 myocardial cells were stimulated by hypoxia and serum-free,and treated with serum containing Huoxue Wentong Formula and blank serum for 4 hours. Cell viability,Caspase 3 and Caspase 8 activities,SOD and CAT activities,MDA content were measured. The changes of ROS were observed by DHE staining,and the apoptotic cells was detected by Hoechst staining. The significant differences among the groups were compared.[Results] Compared with the control group,hypoxic group H9c2 myocardial cell viability,the activities of SOD and CAT decreased significantly (P<0.01),Caspase 3 and Caspase 8 activities,MDA content increased significantly (P<0.01),while the cumulative ROS content and the number of cell apoptosis were increased markedly (P<0.01). However,the serum containing Huoxue Wentong Formula could increase notablely cell viability,SOD and CAT activities (P<0.01),reduce MDA level,Caspase 3 and Caspase 8 activities significantly (P<0.01),and alleviate ROS accumulation,inhibit cardiomyocyte apoptosis (P<0.01).[Conclusion] Huoxue Wentong Formula protected myocardial cells from hypoxia injury to a certain extent by inhibiting apoptosis and alleviating oxidative stress. |
|
|
|
|
|
|
|
