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| Exploration of the mechanism of Compound Angelica Injection on mimicischemic neurons from PI3K/Akt and MAPK/Erk signal pathways |
| Hits 1222 Download times 779 Received:November 30, 2019 |
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| DOI
10.11656/j.issn.1672-1519.2020.04.20 |
| Key Words
mimic ischemia injury;neuron;Compound Argelica Injection;PI3K/Akt;MAPK/Erk;BMECs;ischemic stroke |
| Author Name | Affiliation | E-mail | | LI Yunxin | College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | TANG Yibo | College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | ZHENG Yanfei | College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | LI Yue | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China | | | YU Xue | College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | ZHANG Qiaohui | College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | CHEN Yafei | College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China | | | BAI Xue | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China | | | MA Ru | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China | | | LIU Zhenquan | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China | lzqbzy@sina.com |
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| Abstract
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| [Objective] To investigate the effect and mechanism of Compound Angelica Injection(CAI) on conditioned medium after mimic ischemic brain microvascular endothelial cells(BMECs) on mimic ischemic injured neurons.[Methods] Primitive culture SD rats'BMECs and cortex neuron were identifiedby immunofluorescence. Normal BMECs conditioned medium (N-CM),mimic ischemic injury BMECs conditioned medium (I-CM) andmimic ischemic injury BMECs with different doses of CAI intervention conditioned medium (IC-CM,L=1.25 μL/mL,M=2.5 μL/mL,H=5 μL/mL) were collected,which applied to normal neurons and mimic ischemic neurons respectively. The activity of neurons in each group was detected by CCK-8 method and the phosphorylation level of Akt in PI3K/Akt signaling pathway (p-Akt/Akt)and Erk in MAPK/Erk signaling pathway (p-Erk/Erk) were detected by western blotting.[Results] Compared with N-CM treatment group,the neuronal activity of I-CM treatment group was significantly decreased (P<0.01). Akt phosphorylation level was decreased (P<0.05),Erk phosphorylation level increased significantly (P<0.01). After CAI intervention,the neuronal activity and Akt phosphorylation level in IC-CML group were higher than that in I-CM group (P<0.05). The activity of neurons and the phosphorylation level of Akt in the IC-CML and IC-CMH groups were significantly higher than those in the I-CM group (P<0.01). The phosphorylation level of Erk in each IC-CM group was not significantly different from that in the I-CM group(P>0.05).[Conclusion] CAI can protect the neurons of mimic ischemic injury by interfering with the endothelial cells of ischemic injury. The protective effect may be correlated with the activation of PI3K/Akt signaling pathway,but has little correlation with MAPK/Erk signaling pathway. |
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