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| Influence of the effective decomposed in Nourishing Spleen Removing Dampness and Eliminating Blood-stasis Decoction on the invasion,migration and apoptosis of the human prostate cancer cells based on the IGF/MMP signaling pathway |
| Hits 1300 Download times 867 Received:December 15, 2019 |
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| DOI
10.11656/j.issn.1672-1519.2020.04.21 |
| Key Words
Nourishing Spleen Removing Dampness and Eliminating Blood-stasis Decoction;prostate cancer;cell proliferation;cell migration |
| Author Name | Affiliation | E-mail | | SUN Binxu | Oncology Department, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | | | CAI Qiliang | The Second Hospital of Tianjin Medical University, Tianjin 300211, China | | | LI Xiaojiang | Oncology Department, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | | | JIA Yingjie | Oncology Department, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | jiayingjie1616@sina.com |
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| Abstract
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| [Objective] On the basis of the clinical efficacy of the Nourishing Spleen Removing Dampness and Eliminating Blood-stasis Decoction,combined with the previous animal experiment research,this paper preliminarily screened the best action of Nourishing Spleen Removing Dampness and Eliminating Blood-stasis Decoction through the proliferation experiment of prostate cancer cells. Based on the IGF/MMP signaling pathway,the mechanism of action was explored to lay a foundation for further research on the mechanism of action of all directions.[Methods] We separated compound prescription and then use MTT method to detect cells proliferation inhibition rate from the Jianpi Fuzheng group (Astragalus Nembranaceus,Acanthopanax,Malaytea Scurfpea fruit),the Huayu Sanjie group (Turmeric,Rhubarb,Cowherb seed) and the Qingre Jiedu group (Lyratum Thunb,Snake Six Valley,Plantain). Then through the PC-3 and Du-145 cells proliferation inhibition rate,we choose the best drug part as the further study. Cell invasion and migration were evaluated by transwell and scratch test. The mRNA transcription levels of SDF-1,IGF-1,MMPS and FN genes mRNA were detected by Real-Time PCR. Western blot was used to detect the effects of the Huayu Sanjie group on the protein expressions of SDF-1,IGF-1,MMPS and FN in prostatic activated stromal cell lines.[Results] Among the different un-prescription groups,the inhibition rate of PC-3 and DU-145 was the highest in the removing stasis group,and it increased from 18.77%,58.89% to 87.30%. With the increase of drug concentration,it shows a dose dependence (P<0.05). In the cell migration experiment,compared with the control group,the number of DU-145 invasion cells was 7.33±2.51,22.00±2.65 (P=0.002),and the number of PC-3 invasion cells was 3.67±0.58,22.67±7.77(P<0.05),with statistically significant differences. The scratch test results showed that the migration distance of PC-3 and DU-145 cells in the removing stasis group was (330.12+13.15),(453.34±42.74) μm,and that of the control group was (525.40±6.58),(759.75±40.88) μm,the P values were 0.01 and 0.018 relatively,and the differences were statistically significant. The expression of Vimentin,SM22 and SMM was significantly decreased in the Huayu Sanjie group,while the expression of Calcyclin was not statistically different from that of the control group. The relative expression levels of IGF-1 and MMP genes in the Huayu Sanjie group were lower than those in the high-dose group. Western blot also showed that the drugs in the Huayu Sanjie group reduced the protein expressions of SDF-1,IGF and MMP.[Conclusion] The Huayu Sanjie drugs group can effectively inhibit the proliferation,invasion and migration of prostate cancer cells,and the mechanism may be related to the down-regulation of IGF/MMP signaling pathway by Huayu Sanjie drugs. |
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